serval:BIB_2939CA1FEC9B
Carbon monoxide analysis method in human blood by Airtight Gas Syringe - Gas Chromatography - Mass Spectrometry (AGS-GC-MS): Relevance for postmortem poisoning diagnosis.
10.1016/j.jchromb.2018.05.019
29793099
000434901500011
Oliverio
S.
author
Varlet
V.
author
article
2018-07-15
2018-07-15
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
1873-376X
1570-0232
journal
1090
81-89
Carbon monoxide is one of the most abundant toxic air pollutants. Symptoms of a CO intoxication are non-specific, leading to a high number of misdiagnosed CO poisoning cases that are missing in the disease statistics. The chemical nature of the molecule makes it difficult to detect for long periods and at low levels, thus requiring a very accurate and sensitive method. Current methods capable of accurate and sensitive analyses are available, however an inconsistency between results and symptoms are frequently reported. Therefore, an improved method for the analysis of carbon monoxide in blood and in the headspace (HS) of the sampling tube with the use of Airtight Gas Syringe - Gas Chromatography - Mass Spectrometry (AGS-GC-MS) is hereby presented and validated, for CO concentrations in a range of 10-200 nmol/mL HS (2-40 μmol/mL blood). Analytical LOQ is found at 0.9 nmol/mL HS (0.18 μmol/mL blood) and LOD at 0.1 nmol/mL gas. Application to intoxicated samples from autopsies and comparison to previously published methods show that this method is more appropriate, since performed under fully controlled conditions. Results show higher CO concentrations compared to previous approaches, indicating that results might have been underestimating the true blood CO burden. Therefore, this approach has the potential to help reduce the misdiagnosed cases and the gap between measurement and diagnosis of CO poisonings.
Adult
Aged
Autopsy
Blood Gas Analysis/methods
Carbon Monoxide/blood
Carboxyhemoglobin/analysis
Female
Forensic Toxicology
Gas Chromatography-Mass Spectrometry/methods
Humans
Limit of Detection
Linear Models
Male
Reproducibility of Results
Young Adult
Airtight Gas Syringe
Analytical measurement
Carbon monoxide
Carboxyhaemoglobin
GC-MS
eng
60_published
peer-reviewed
Publication types: Journal Article
Publication Status: ppublish
University of Lausanne
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