serval:BIB_22A6580B8935
Blunting the response to endotoxin in healthy subjects: effects of various doses of intravenous fish oil.
10.1007/s00134-009-1689-8
000273810500015
19844694
Pittet
Y.K.
author
Berger
M.M.
author
Pluess
T.T.
author
Voirol
P.
author
Revelly
J.P.
author
Tappy
L.
author
Chioléro
R.L.
author
article
2010-02
Intensive care medicine
1432-1238
0342-4642
journal
36
2
289-295
To test the dose response effect of infused fish oil (FO) rich in n-3 PUFAs on the inflammatory response to endotoxin (LPS) and on membrane incorporation of fatty acids in healthy subjects.
Prospective, sequential investigation comparing three different FO doses.
Three groups of male subjects aged 26.8 +/- 3.2 years (BMI 22.5 +/- 2.1).
One of three FO doses (Omegaven10%) as a slow infusion before LPS: 0.5 g/kg 1 day before LPS, 0.2 g/kg 1 day before, or 0.2 g/kg 2 h before.
Temperature, hemodynamic variables, indirect calorimetry and blood samples (TNF-alpha, stress hormones) were collected. After LPS temperature, ACTH and TNF-alpha concentrations increased in the three groups: the responses were significantly blunted (p < 0.0001) compared with the control group of the Pluess et al. trial. Cortisol was unchanged. Lowest plasma ACTH, TNF-alpha and temperature AUC values were observed after a single 0.2 g/kg dose of FO. EPA incorporation into platelet membranes was dose-dependent.
Having previously shown that the response to LPS was reproducible, this study shows that three FO doses blunted it to various degrees. The 0.2 g/kg perfusion immediately before LPS was the most efficient in blunting the responses, suggesting LPS capture in addition to the systemic and membrane effects.
Adolescent
Adrenocorticotropic Hormone/blood
Adrenocorticotropic Hormone/drug effects
Adult
Calorimetry, Indirect
Endotoxins/antagonists & inhibitors
Endotoxins/metabolism
Fish Oils/administration & dosage
Fish Oils/pharmacology
Fish Oils/therapeutic use
Humans
Infusions, Intravenous
Male
Prospective Studies
Sepsis/metabolism
Sepsis/therapy
Time Factors
Tumor Necrosis Factor-alpha/metabolism
Young Adult
eng
60_published
true
peer-reviewed
Publication types: Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
University of Lausanne
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