serval:BIB_1793CE3D7607
In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system.
10.1038/s41467-017-01419-1
000414229000003
29093476
Petit-Pierre
G.
author
Colin
P.
author
Laurer
E.
author
Déglon
J.
author
Bertsch
A.
author
Thomas
A.
author
Schneider
B.L.
author
Renaud
P.
author
article
2017-11-01
Nature communications
2041-1723
2041-1723
journal
8
1
1239
Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. Current approaches using microdialysis catheters are however drastically limited in term of temporal resolution. Here we show a functional in vivo validation of a droplet collection system included at the tip of a neural probe. The system comprises an advanced droplet formation mechanism which enables the collection of neurochemicals present in the brain ECF at high-temporal resolution. The probe was implanted in a rat brain and could successfully collect fluid samples organized in a train of droplets. A microfabricated target plate compatible with most of the surface-based detection methods was specifically developed for sample analysis. The time-resolved brain-fluid samples are analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The results provide a time evolution picture of the cerebral tissues neurochemical composition for selected elements known for their involvement in neurodegenerative diseases.
Animals
Brain
Brain Chemistry
Calcium/analysis
Cerebrum/chemistry
Extracellular Fluid/chemistry
Female
Magnesium/analysis
Mass Spectrometry
Mercury/analysis
Microdialysis/methods
Potassium/analysis
Rats
Rats, Sprague-Dawley
Sodium/analysis
Time Factors
eng
60_published
true
peer-reviewed
Publication types: Journal Article
Publication Status: epublish
University of Lausanne
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