serval:BIB_13116D04E8C9
Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum.
10.1038/ismej.2016.23
000386664600006
26953603
de Barsy
M.
author
Frandi
A.
author
Panis
G.
author
Théraulaz
L.
author
Pillonel
T.
author
Greub
G.
author
Viollier
P.H.
author
article
2016-09
The ISME journal
1751-7370
1751-7362
journal
10
9
2129-2144
Like other obligate intracellular bacteria, the Chlamydiae feature a compact regulatory genome that remains uncharted owing to poor genetic tractability. Exploiting the reduced number of transcription factors (TFs) encoded in the chlamydial (pan-)genome as a model for TF control supporting the intracellular lifestyle, we determined the conserved landscape of TF specificities by ChIP-Seq (chromatin immunoprecipitation-sequencing) in the chlamydial pathogen Waddlia chondrophila. Among 10 conserved TFs, Euo emerged as a master TF targeting >100 promoters through conserved residues in a DNA excisionase-like winged helix-turn-helix-like (wHTH) fold. Minimal target (Euo) boxes were found in conserved developmentally-regulated genes governing vertical genome transmission (cytokinesis and DNA replication) and genome plasticity (transposases). Our ChIP-Seq analysis with intracellular bacteria not only reveals that global TF regulation is maintained in the reduced regulatory genomes of Chlamydiae, but also predicts that master TFs interpret genomic information in the obligate intracellular α-proteobacteria, including the rickettsiae, from which modern day mitochondria evolved.
Animals
Bacterial Proteins/genetics
Cercopithecus aethiops
Chlamydiales/genetics
Chromatin Immunoprecipitation
Genome, Bacterial/genetics
Genomics
Phylogeny
Reproducibility of Results
Transcription Factors/genetics
Vero Cells
Verrucomicrobia/genetics
eng
60_published
true
peer-reviewed
Publication types: Journal Article
Publication Status: ppublish
University of Lausanne
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