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2024-03-23T03:09:56Z
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Microcolony growth assays
10.1007/978-3-540-77587-4_279
Reinhard
F.
author
van der Meer
J. R.
author
incollection
chapter
2010
Springer
Berlin
Handbook of hydrocarbon and lipid microbiology
Timmis
K. N.
editor
978-3-540-77584-3
Springer Reference
book
5
3562-3570
16
eng
60_published
Microcolony growth procedures have gained recent interest for the following reasons: rapid simultaneous screening for growth of large numbers of bacteria from environmental samples, time-lapse imaging of single-cell growth, cell aging or biofilm studies. Not many of such procedures have been described in sufficient technical detail to be easily reproduced. Here we provide a simple step-by-step procedure, which allows time-lapse imaging of bacterial cell division and cell tracking up to the stage of a monolayered colony of a few 100 cells.