Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae.

Details

Serval ID
serval:BIB_FC107F826A5D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae.
Journal
Diagnostic microbiology and infectious disease
Author(s)
Jayol A., Kieffer N., Poirel L., Guérin F., Güneser D., Cattoir V., Nordmann P.
ISSN
1879-0070 (Electronic)
ISSN-L
0732-8893
Publication state
Published
Issued date
10/2018
Peer-reviewed
Oui
Volume
92
Number
2
Pages
90-94
Language
english
Notes
Publication types: Evaluation Studies ; Journal Article
Publication Status: ppublish
Abstract
The commercial Rapid Polymyxin NP test was evaluated to detect colistin-resistant Enterobacteriaceae. A total of 223 enterobacterial isolates corresponding to 136 resistant (including 38 MCR-like producers), 19 heteroresistant, and 78 colistin-susceptible isolates were tested. The test was performed according to the manufacturer's instruction, and the color of the wells was read after 2 and 3 hours of incubation. The results were compared with those of the homemade Rapid Polymyxin NP test, and manual broth microdilution according to EUCAST guidelines was used as the reference method to determine the performance of the test. Excellent performance of the commercial Rapid Polymyxin NP test was found with a very major error rate, a major error rate, a sensitivity, and a specificity of 1.9%, 5.1%, 98.1%, and 94.9%, respectively. The performance of the homemade Polymyxin NP test was similar, with a slightly better value for the very major error (1.2 %).
Keywords
Anti-Bacterial Agents/pharmacology, Colistin/pharmacology, Drug Resistance, Bacterial, Enterobacteriaceae/drug effects, Enterobacteriaceae/isolation & purification, Enterobacteriaceae Infections/microbiology, Humans, Microbial Sensitivity Tests/methods, Polymyxins/pharmacology, Reagent Kits, Diagnostic, Sensitivity and Specificity, Time Factors, Enterobacteriaceae, MCR, Polymyxin, Rapid test
Pubmed
Web of science
Create date
08/10/2018 16:35
Last modification date
20/08/2019 16:27
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