Absolute Quantification of Noncoding RNA by Microscale Thermophoresis.

Détails

ID Serval
serval:BIB_FBBD909C9238
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Absolute Quantification of Noncoding RNA by Microscale Thermophoresis.
Périodique
Angewandte Chemie
Auteur(s)
Jacob D., Thüring K., Galliot A., Marchand V., Galvanin A., Ciftci A., Scharmann K., Stock M., Roignant J.Y., Leidel S.A., Motorin Y., Schaffrath R., Klassen R., Helm M.
ISSN
1521-3773 (Electronic)
ISSN-L
1433-7851
Statut éditorial
Publié
Date de publication
08/07/2019
Peer-reviewed
Oui
Volume
58
Numéro
28
Pages
9565-9569
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization-based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA-Seq-based quantification approaches, strongly suggesting a bias due to tRNA modifications in the latter. Further applications include the quantification of rRNA as well as of polyA levels in cellular RNA.
Mots-clé
RNA quantification, fluorescence, hybridization, microscale thermophoresis, tRNA stability
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/10/2019 12:26
Dernière modification de la notice
29/10/2019 6:26
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