Blood-Borne Circadian Signal Stimulates Daily Oscillations in Actin Dynamics and SRF Activity.

Details

Serval ID
serval:BIB_FACDD93BEF82
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Blood-Borne Circadian Signal Stimulates Daily Oscillations in Actin Dynamics and SRF Activity.
Journal
Cell
Author(s)
Gerber A., Esnault C., Aubert G., Treisman R., Pralong F., Schibler U.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Publication state
Published
Issued date
2013
Volume
152
Number
3
Pages
492-503
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish PDF type: Article
Abstract
In peripheral tissues circadian gene expression can be driven either by local oscillators or by cyclic systemic cues controlled by the master clock in the brain's suprachiasmatic nucleus. In the latter case, systemic signals can activate immediate early transcription factors (IETFs) and thereby control rhythmic transcription. In order to identify IETFs induced by diurnal blood-borne signals, we developed an unbiased experimental strategy, dubbed Synthetic TAndem Repeat PROMoter (STAR-PROM) screening. This technique relies on the observation that most transcription factor binding sites exist at a relatively high frequency in random DNA sequences. Using STAR-PROM we identified serum response factor (SRF) as an IETF responding to oscillating signaling proteins present in human and rodent sera. Our data suggest that in mouse liver SRF is regulated via dramatic diurnal changes of actin dynamics, leading to the rhythmic translocation of the SRF coactivator Myocardin-related transcription factor-B (MRTF-B) into the nucleus.
Pubmed
Web of science
Open Access
Yes
Create date
01/03/2013 18:02
Last modification date
20/08/2019 16:26
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