Screening for wound-induced oxylipins in Arabidopsis thaliana by differential HPLC-APCI/MS profiling of crude leaf extracts and subsequent characterisation by capillary-scale NMR

Détails

ID Serval
serval:BIB_FA79B8472706
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Screening for wound-induced oxylipins in Arabidopsis thaliana by differential HPLC-APCI/MS profiling of crude leaf extracts and subsequent characterisation by capillary-scale NMR
Périodique
Phytochemical Analysis
Auteur(s)
Thiocone A., Farmer E. E., Wolfender J. L.
ISSN
1099-1565
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
19
Numéro
3
Pages
198-205
Langue
anglais
Résumé
A simple non-targeted differential HPLC-APCI/MS approach has been developed in order to survey metabolome modifications that occur in the leaves of Arabidopsis thaliana following wound-induced stress. The wound-induced accumulation of metabolites, particularly oxylipins, was evaluated by HPLC-MS analysis of crude leaf extracts. A generic, rapid and reproducible pressure liquid extraction procedure was developed for the analysis of restricted leaf samples without the need for specific sample preparation. The presence of various oxylipins was determined by head-to-head comparison of the HPLC-MS data, filtered with a component detection algorithm, and automatically compared with the aid of software searching for small differences in similar HPLC-MS profiles. Repeatability was verified in several specimens belonging to different series. Wound-inducible jasmonates were efficiently highlighted by this non-targeted approach without the need for complex sample preparation as is the case for the 'oxylipin signature' procedure based on GC-MS. Furthermore this HPLC-MS screening technique allowed the isolation of induced compounds for further characterisation by capillary-scale NMR (CapNMR) after HPLC scale-up. In this paper, the screening method is described and applied to illustrate its potential for monitoring polar and non-polar stress-induced constituents as well as its use in combination with CapNMR for the structural assignment of wound-induced compounds of interest
Mots-clé
analysis , Arabidopsis , chemistry , Chromatography,High Pressure Liquid , Gene Expression Profiling , Gene Expression Regulation,Plant , Magnetic Resonance Spectroscopy , Mass Spectrometry , metabolism , methods , Molecular Structure , Oxylipins , physiology , Plant Extracts , Plant Leaves , Switzerland
Pubmed
Web of science
Création de la notice
29/01/2009 23:14
Dernière modification de la notice
03/03/2018 22:56
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