The CaMV 35S promoter has a weak expression activity in dark grown tissues of moss Physcomitrella patens

Details

Serval ID
serval:BIB_F95A9A9239F2
Type
Article: article from journal or magazin.
Collection
Publications
Title
The CaMV 35S promoter has a weak expression activity in dark grown tissues of moss Physcomitrella patens
Journal
Plant Signal Behav
Author(s)
Saidi Younousse, Schaefer Didier G, Goloubinoff Pierre, Zrÿd Jean-Pierre, Finka Andrija
Publication state
Published
Issued date
05/2009
Peer-reviewed
Oui
Volume
4
Number
5
Pages
457-459
Language
english
Abstract
The constitutive Cauliflower Mosaic Virus 35S promoter (CaMV 35S) is widely used as a tool to express recombinant proteins in plants, but with different success. We previously showed that the expression of an F-actin marker, GFP-talin, in Physcomitrella patens using the CaMV 35S promoter failed to homogenously label moss tissues. Here, we show a significant diminution of the GFP fluorescence in dark grown old moss cells and complete lack of labelling in newly differentiated cells. Furthermore, we demonstrate that stable moss lines harbouring a resistance cassette driven by the CaMV 35S are unable to grow in darkness in the presence of the antibiotic. In contrast to the CaMV 35S, the heat inducible promoter, hsp17.3B showed uniform expression pattern in all cells and tissues following a mild heat shock.
Keywords
F-actin, constitutive/inducible promoter, heat shock, hsp17.3B, sodium salicylate
Create date
06/01/2011 12:06
Last modification date
14/07/2020 14:04
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