A sensitive and specific two-site, sandwich-amplified enzyme immunoassay for neuropeptide Y.

Détails

ID Serval
serval:BIB_F8F8486ED311
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A sensitive and specific two-site, sandwich-amplified enzyme immunoassay for neuropeptide Y.
Périodique
Peptides
Auteur(s)
Grouzmann E., Aubert J.F., Waeber B., Brunner H.R.
ISSN
0196-9781
Statut éditorial
Publié
Date de publication
1992
Peer-reviewed
Oui
Volume
13
Numéro
6
Pages
1049-1054
Langue
anglais
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Nov-Dec
Résumé
The development of a new enzyme immunoassay for neuropeptide Y (NPY) is reported. Two monoclonal antibodies directed against distinct epitopes of NPY are used, one as a capture antibody (NPY02) and the other one as an indicator antibody (NPY05), this latter antibody being labeled with alkaline phosphatase. The assay calibration curve was performed over concentrations of 1 to 250 pM in a NPY-free plasma. The intra-assay coefficient of variation (CV) ranged from 0.025 to 11.9%, whereas the interassay CV was comprised between 5 and 12%. The limit of detection of this assay was 1 pM (100 amol/well). Neuropeptide Y levels are related to sampling conditions; basal concentrations of NPY with low SEM are found when less than 1.2 ml of blood is taken in EDTA tubes, the sample is centrifuged at 4 degrees C, and immediately frozen. Unanesthetized spontaneously hypertensive rats exhibited higher NPY plasma concentrations than normotensive Wistar-Kyoto controls (53 +/- 7 pM and 25 +/- 2 pM, respectively, mean +/- SEM, p < 0.01). Plasma NPY levels are similar in 16- and 36-week-old animals. In conclusion, this technique makes it possible to assay a large number of samples within 24 h without requiring radioactivity.
Mots-clé
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Hypertension, Immunoenzyme Techniques, Molecular Sequence Data, Neuropeptide Y, Rats, Rats, Inbred SHR, Rats, Inbred WKY, Sensitivity and Specificity
Pubmed
Web of science
Création de la notice
25/01/2008 11:55
Dernière modification de la notice
03/03/2018 22:53
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