A sensitive and specific two-site, sandwich-amplified enzyme immunoassay for neuropeptide Y.

Details

Serval ID
serval:BIB_F8F8486ED311
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A sensitive and specific two-site, sandwich-amplified enzyme immunoassay for neuropeptide Y.
Journal
Peptides
Author(s)
Grouzmann E., Aubert J.F., Waeber B., Brunner H.R.
ISSN
0196-9781
Publication state
Published
Issued date
1992
Peer-reviewed
Oui
Volume
13
Number
6
Pages
1049-1054
Language
english
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Nov-Dec
Abstract
The development of a new enzyme immunoassay for neuropeptide Y (NPY) is reported. Two monoclonal antibodies directed against distinct epitopes of NPY are used, one as a capture antibody (NPY02) and the other one as an indicator antibody (NPY05), this latter antibody being labeled with alkaline phosphatase. The assay calibration curve was performed over concentrations of 1 to 250 pM in a NPY-free plasma. The intra-assay coefficient of variation (CV) ranged from 0.025 to 11.9%, whereas the interassay CV was comprised between 5 and 12%. The limit of detection of this assay was 1 pM (100 amol/well). Neuropeptide Y levels are related to sampling conditions; basal concentrations of NPY with low SEM are found when less than 1.2 ml of blood is taken in EDTA tubes, the sample is centrifuged at 4 degrees C, and immediately frozen. Unanesthetized spontaneously hypertensive rats exhibited higher NPY plasma concentrations than normotensive Wistar-Kyoto controls (53 +/- 7 pM and 25 +/- 2 pM, respectively, mean +/- SEM, p < 0.01). Plasma NPY levels are similar in 16- and 36-week-old animals. In conclusion, this technique makes it possible to assay a large number of samples within 24 h without requiring radioactivity.
Keywords
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Hypertension, Immunoenzyme Techniques, Molecular Sequence Data, Neuropeptide Y, Rats, Rats, Inbred SHR, Rats, Inbred WKY, Sensitivity and Specificity
Pubmed
Web of science
Create date
25/01/2008 10:55
Last modification date
20/08/2019 16:24
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