Article: article from journal or magazin.
New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples
Journal of Clinical Microbiology
Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.
Acanthamoeba , Adolescent , analysis , Animals , Bacteria , Bronchiolitis , Child,Preschool , Chlamydiales , diagnosis , Dna , DNA,Bacterial , Female , genetics , Gram-Negative Bacterial Infections , Humans , Infant , isolation & purification , Male , methods , microbiology , Patients , Polymerase Chain Reaction , Reproducibility of Results , Role , Sensitivity and Specificity , Switzerland
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