New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples

Details

Serval ID
serval:BIB_F8EF3D970451
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples
Journal
Journal of Clinical Microbiology
Author(s)
Casson N., Posfay-Barbe K. M., Gervaix A., Greub G.
ISSN
1098-660X
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
46
Number
4
Pages
1491-1493
Language
english
Abstract
Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.
Keywords
Acanthamoeba , Adolescent , analysis , Animals , Bacteria , Bronchiolitis , Child,Preschool , Chlamydiales , diagnosis , Dna , DNA,Bacterial , Female , genetics , Gram-Negative Bacterial Infections , Humans , Infant , isolation & purification , Male , methods , microbiology , Patients , Polymerase Chain Reaction , Reproducibility of Results , Role , Sensitivity and Specificity , Switzerland
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2009 22:13
Last modification date
20/08/2019 16:24
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