Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4

Détails

ID Serval
serval:BIB_F8E1496A0CC1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4
Périodique
Development
Auteur(s)
Hardtke  C. S., Ckurshumova  W., Vidaurre  D. P., Singh  S. A., Stamatiou  G., Tiwari  S. B., Hagen  G., Guilfoyle  T. J., Berleth  T.
ISSN
0950-1991 (Print)
Statut éditorial
Publié
Date de publication
03/2004
Volume
131
Numéro
5
Pages
1089-100
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
Transcription factors of the auxin response factor (ARF) family have been implicated in auxin-dependent gene regulation, but little is known about the functions of individual ARFs in plants. Here, interaction assays, expression studies and combinations of multiple loss- and gain-of-function mutants were used to assess the roles of two ARFs, NONPHOTOTROPIC HYPOCOTYL 4 (NPH4/ARF7) and MONOPTEROS (MP/ARF5), in Arabidopsis development. Both MP and NPH4 interact strongly and selectively with themselves and with each other, and are expressed in vastly overlapping domains. We show that the regulatory properties of both genes are far more related than suggested by their single mutant phenotypes. NPH4 and MP are capable of controlling both axis formation in the embryo and auxin-dependent cell expansion. Interaction of MP and NPH4 in Arabidopsis plants is indicated by their joint requirement in a number of auxin responses and by synergistic effects associated with the co-overexpression of both genes. Finally, we demonstrate antagonistic interaction between ARF and Aux/IAA gene functions in Arabidopsis development. Overexpression of MP suppresses numerous defects associated with a gain-of-function mutation in BODENLOS (BDL)/IAA12. Together these results provide evidence for the biological relevance of ARF-ARF and ARF-Aux/IAA interaction in Arabidopsis plants and demonstrate that an individual ARF can act in both invariantly programmed pattern formation as well as in conditional responses to external signals.
Mots-clé
Arabidopsis/genetics/*growth & development/*physiology Arabidopsis Proteins/genetics/*physiology Base Sequence Body Patterning DNA, Plant/genetics DNA-Binding Proteins/genetics/*physiology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant In Situ Hybridization Indoleacetic Acids/physiology Mutation Phenotype Plants, Genetically Modified RNA, Messenger/genetics/metabolism RNA, Plant/genetics/metabolism Transcription Factors/genetics/*physiology Two-Hybrid System Techniques
Pubmed
Web of science
Création de la notice
24/01/2008 20:51
Dernière modification de la notice
03/03/2018 22:53
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