Deoxyribonucleic acid-protein interactions and expression of the human testis-specific lactate dehydrogenase promoter: transcription factor Sp1 plays a major role

Détails

ID Serval
serval:BIB_F8B6EA9537BE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Deoxyribonucleic acid-protein interactions and expression of the human testis-specific lactate dehydrogenase promoter: transcription factor Sp1 plays a major role
Périodique
Biology of Reproduction
Auteur(s)
Bonny  C., Cooker  L. A., Goldberg  E.
ISSN
0006-3363 (Print)
Statut éditorial
Publié
Date de publication
03/1998
Volume
58
Numéro
3
Pages
754-9
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Résumé
The human testis-specific lactate dehydrogenase c gene (ldh-c) shows an exceptionally large window of expression throughout pre- and postmeiotic stages of the male germ cell lineage. In order to characterize the multiple stage-specific transcription factors necessary for ldh-c expression, we previously characterized the human ldh-c core promoter. Here, we used a combination of gel retardation assays and an in vitro transcription system derived from human tissues to better define the elements that govern ldh-c transcription. Three classes of transcriptional regulators were defined by these experiments. 1) The Sp1 transcription factor is a testis-"enriched" protein that is absent from most somatic tissues and that appears to play a major role in determining ldh-c expression in the testis. Highest levels of Sp1 during spermatogenesis correlate with maxima of ldh-c expression. 2) The testis-specific cAMP response element modulator (CREM) transcription factor binds a cAMP response element (CRE)-like sequence located at position -433. This transcriptional activator might contribute to postmeiotic transcription of ldh-c. 3) Factors present in tissues negative for ldh-c expression appear to bind both the CRE-like sequence and an adjacent hormone response element. The presence of this element could be involved in regulating ldh-c through the glucocorticoid/androgen pathways at the early stages of ldh-c expression.
Mots-clé
Base Sequence Cyclic AMP Response Element Modulator DNA/*genetics/*metabolism DNA-Binding Proteins/genetics/metabolism Gene Expression Humans L-Lactate Dehydrogenase/*genetics Male Molecular Sequence Data *Promoter Regions (Genetics) *Repressor Proteins Sp1 Transcription Factor/*metabolism Spermatogenesis/genetics/physiology Testis/*metabolism Tissue Distribution Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:16
Dernière modification de la notice
09/05/2019 3:42
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