Cellular entry of lymphocytic choriomeningitis virus.

Détails

ID Serval
serval:BIB_F7F2FDBFE326
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cellular entry of lymphocytic choriomeningitis virus.
Périodique
Journal of Virology
Auteur(s)
Rojek J.M., Perez M., Kunz S.
ISSN
1098-5514 (Electronic)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
2008
Volume
82
Numéro
3
Pages
1505-1517
Langue
anglais
Résumé
In contrast to most enveloped viruses that enter the host cell via clathrin-dependent endocytosis, the Old World arenavirus lymphocytic choriomeningitis virus (LCMV) enters cells via noncoated vesicles that deliver the virus to endosomes, where pH-dependent membrane fusion occurs. Here, we investigated the initial steps of LCMV infection. We found that the attachment of LCMV to its cellular receptor alpha-dystroglycan occurs rapidly and is not dependent on membrane cholesterol. However, subsequent virus internalization is sensitive to cholesterol depletion, indicating the involvement of a cholesterol-dependent pathway. We provide evidence that LCMV entry involves an endocytotic pathway that is independent of clathrin and caveolin and that does not require the GTPase dynamin. In addition, neither the structural integrity nor the dynamics of the actin cytoskeleton are required for infection. These findings indicate that the prototypic Old World arenavirus LCMV uses a mechanism of entry that is different from clathrin-mediated endocytosis, which is used by the New World arenavirus Junin virus, and pathways used by other enveloped viruses.
Mots-clé
Actins/metabolism, Animals, Caveolins/metabolism, Cell Line, Cell Membrane/chemistry, Cercopithecus aethiops, Cholesterol/physiology, Clathrin/metabolism, Cricetinae, Dynamins/metabolism, Dystroglycans/metabolism, Endocytosis/physiology, Humans, Lymphocytic choriomeningitis virus/physiology, Mesocricetus, Virus Attachment, Virus Internalization
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/04/2013 12:56
Dernière modification de la notice
09/05/2019 3:40
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