Cellular entry of lymphocytic choriomeningitis virus.

Details

Serval ID
serval:BIB_F7F2FDBFE326
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cellular entry of lymphocytic choriomeningitis virus.
Journal
Journal of Virology
Author(s)
Rojek J.M., Perez M., Kunz S.
ISSN
1098-5514 (Electronic)
ISSN-L
0022-538X
Publication state
Published
Issued date
2008
Volume
82
Number
3
Pages
1505-1517
Language
english
Abstract
In contrast to most enveloped viruses that enter the host cell via clathrin-dependent endocytosis, the Old World arenavirus lymphocytic choriomeningitis virus (LCMV) enters cells via noncoated vesicles that deliver the virus to endosomes, where pH-dependent membrane fusion occurs. Here, we investigated the initial steps of LCMV infection. We found that the attachment of LCMV to its cellular receptor alpha-dystroglycan occurs rapidly and is not dependent on membrane cholesterol. However, subsequent virus internalization is sensitive to cholesterol depletion, indicating the involvement of a cholesterol-dependent pathway. We provide evidence that LCMV entry involves an endocytotic pathway that is independent of clathrin and caveolin and that does not require the GTPase dynamin. In addition, neither the structural integrity nor the dynamics of the actin cytoskeleton are required for infection. These findings indicate that the prototypic Old World arenavirus LCMV uses a mechanism of entry that is different from clathrin-mediated endocytosis, which is used by the New World arenavirus Junin virus, and pathways used by other enveloped viruses.
Keywords
Actins/metabolism, Animals, Caveolins/metabolism, Cell Line, Cell Membrane/chemistry, Cercopithecus aethiops, Cholesterol/physiology, Clathrin/metabolism, Cricetinae, Dynamins/metabolism, Dystroglycans/metabolism, Endocytosis/physiology, Humans, Lymphocytic choriomeningitis virus/physiology, Mesocricetus, Virus Attachment, Virus Internalization
Pubmed
Web of science
Open Access
Yes
Create date
17/04/2013 11:56
Last modification date
20/08/2019 16:24
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