Article: article from journal or magazin.
Construction and characterization of a recombinant adenovirus directing expression of the MAGE-1 tumor-specific antigen
International Journal of Cancer
Journal Article --- Old month value: Sep 17
The finding that many human melanomas express distinct antigens that can be recognised by specific cytolytic T lymphocytes (CTL) implies that immunotherapeutic strategies against this cancer might prove effective. The ex vivo delivery of a tumour-associated antigen to autologous cells and the subsequent re-administration of these cells to the patient might prove effective in boosting the T cell immune response. Recombinant human adenoviral vectors provide an efficient delivery system and have many advantages over other viral and non-viral delivery vehicles. Infection of a panel of human melanoma cell lines by AdCMVMAGE-1, a novel recombinant adenovirus which incorporates the full-length MAGE-1 cDNA, was shown to induce production of high levels of MAGE-1 protein. Incubation of transduced HLA-A1 expressing melanoma cell lines with 2 anti-MAGE-1.A1 CTL clones resulted in specific recognition and lysis of target cells, indicating that the exogenous MAGE-1 protein was processed and presented in a normal manner. Furthermore, quantitative analyses demonstrated a correlation between the efficiency of transduction and the proportion of cells lysed. Importantly for future clinical trials, stimulation of peripheral blood lymphocytes (PBLs) from a melanoma patient by AdCMVMAGE-1-transduced autologous cells resulted in the generation of specific CTLs against the MAGE-1 antigen. Together, our data emphasize the utility of adenoviruses as vaccination vehicles and highlight the potential efficacy of this approach for the treatment of melanoma.
Adenoviridae/*genetics Antigens, Neoplasm/*biosynthesis Cytotoxicity, Immunologic Genetic Vectors Humans Melanoma/*immunology Neoplasm Proteins/*biosynthesis/immunology Recombinant Fusion Proteins/biosynthesis Recombination, Genetic T-Lymphocytes, Cytotoxic/*immunology Transfection/methods Tumor Cells, Cultured beta-Galactosidase/biosynthesis
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