Construction and characterization of a recombinant adenovirus directing expression of the MAGE-1 tumor-specific antigen

Détails

ID Serval
serval:BIB_F7E97F816738
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Construction and characterization of a recombinant adenovirus directing expression of the MAGE-1 tumor-specific antigen
Périodique
International Journal of Cancer
Auteur(s)
Reed  D. S., Romero  P., Rimoldi  D., Cerottini  J. C., Schaack  J., Jongeneel  C. V.
ISSN
0020-7136 (Print)
Statut éditorial
Publié
Date de publication
09/1997
Volume
72
Numéro
6
Pages
1045-55
Notes
Journal Article --- Old month value: Sep 17
Résumé
The finding that many human melanomas express distinct antigens that can be recognised by specific cytolytic T lymphocytes (CTL) implies that immunotherapeutic strategies against this cancer might prove effective. The ex vivo delivery of a tumour-associated antigen to autologous cells and the subsequent re-administration of these cells to the patient might prove effective in boosting the T cell immune response. Recombinant human adenoviral vectors provide an efficient delivery system and have many advantages over other viral and non-viral delivery vehicles. Infection of a panel of human melanoma cell lines by AdCMVMAGE-1, a novel recombinant adenovirus which incorporates the full-length MAGE-1 cDNA, was shown to induce production of high levels of MAGE-1 protein. Incubation of transduced HLA-A1 expressing melanoma cell lines with 2 anti-MAGE-1.A1 CTL clones resulted in specific recognition and lysis of target cells, indicating that the exogenous MAGE-1 protein was processed and presented in a normal manner. Furthermore, quantitative analyses demonstrated a correlation between the efficiency of transduction and the proportion of cells lysed. Importantly for future clinical trials, stimulation of peripheral blood lymphocytes (PBLs) from a melanoma patient by AdCMVMAGE-1-transduced autologous cells resulted in the generation of specific CTLs against the MAGE-1 antigen. Together, our data emphasize the utility of adenoviruses as vaccination vehicles and highlight the potential efficacy of this approach for the treatment of melanoma.
Mots-clé
Adenoviridae/*genetics Antigens, Neoplasm/*biosynthesis Cytotoxicity, Immunologic Genetic Vectors Humans Melanoma/*immunology Neoplasm Proteins/*biosynthesis/immunology Recombinant Fusion Proteins/biosynthesis Recombination, Genetic T-Lymphocytes, Cytotoxic/*immunology Transfection/methods Tumor Cells, Cultured beta-Galactosidase/biosynthesis
Pubmed
Web of science
Création de la notice
24/01/2008 16:39
Dernière modification de la notice
03/03/2018 22:51
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