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Phenotype-dependent inhibition of human alpha 1-antitrypsin by 1,6-hexane diamine in vitro
In Vitro Journal Article --- Old month value: Jul
Blood donors and patients from pulmonary wards gave serum specimens for the assay of their alpha 1-antitrypsin activity and phenotype. The same specimens were then incubated at the room temperature overnight with increasing concentrations of 1,6-hexane diamine. The 5 mM amine concentration caused a significant decrease in heterozygous antitrypsins (M1M2, M1M3, M2M3 and M1S) activity while it was less in the homozygous (M1M1 and M2M2) antitrypsin phenotypes. The SS and ZZ phenotypes showed a very low initial activity which was, however, further reduced. Analysis for the antitrypsin protein showed a simultaneous loss of its activity. The data suggest that heterozygous antitrypsin carriers may be more prone to the effects of inhaled amines despite their 'normal' phenotypic constitution.
Diamines/*pharmacology Heterozygote Homozygote Humans Isoelectric Focusing Phenotype alpha 1-Antitrypsin/*genetics
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