On the role of type IX collagen in the extracellular matrix of cartilage: type IX collagen is localized to intersections of collagen fibrils.

Details

Serval ID
serval:BIB_F496ECE106BE
Type
Article: article from journal or magazin.
Collection
Publications
Title
On the role of type IX collagen in the extracellular matrix of cartilage: type IX collagen is localized to intersections of collagen fibrils.
Journal
Journal of Cell Biology
Author(s)
Müller-Glauser W., Humbel B., Glatt M., Sträuli P., Winterhalter K.H., Bruckner P.
ISSN
0021-9525 (Print)
ISSN-L
0021-9525
Publication state
Published
Issued date
1986
Volume
102
Number
5
Pages
1931-1939
Language
english
Abstract
The tissue distribution of type II and type IX collagen in 17-d-old chicken embryo was studied by immunofluorescence using polyclonal antibodies against type II collagen and a peptic fragment of type IX collagen (HMW), respectively. Both proteins were found only in cartilage where they were co-distributed. They occurred uniformly throughout the extracellular matrix, i.e., without distinction between pericellular, territorial, and interterritorial matrices. Tissues that undergo endochondral bone formation contained type IX collagen, whereas periosteal and membranous bones were negative. The thin collagenous fibrils in cartilage consisted of type II collagen as determined by immunoelectron microscopy. Type IX collagen was associated with the fibrils but essentially was restricted to intersections of the fibrils. These observations suggested that type IX collagen contributes to the stabilization of the network of thin fibers of the extracellular matrix of cartilage by interactions of its triple helical domains with several fibrils at or close to their intersections.
Keywords
Animals, Cartilage/ultrastructure, Chick Embryo, Collagen/physiology, Extracellular Matrix/ultrastructure, Fluorescent Antibody Technique, Gold/diagnostic use, Microscopy, Electron/methods, Tissue Distribution
Pubmed
Web of science
Open Access
Yes
Create date
18/10/2012 13:02
Last modification date
20/08/2019 16:21
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