Integrated fluorescence and transmission electron microscopy: A novel approach to correlate microscopy

Details

Serval ID
serval:BIB_F4654F9C4BCF
Type
Article: article from journal or magazin.
Collection
Publications
Title
Integrated fluorescence and transmission electron microscopy: A novel approach to correlate microscopy
Journal
Journal of Structural Biology
Author(s)
Agronskaia A.V., Valentijn J.A., van Driel L.F., Schneijdenberg C.T.W.M., Humbel B.M., van Bergen en Henegouwen P.M.P., Verkleij A.J., Koster A.J., Gerritsen H.C.
ISSN
1095-8657 (Electronic)
ISSN-L
1047-8477
Publication state
Published
Issued date
2008
Volume
164
Number
2
Pages
183-189
Language
english
Abstract
Correlative microscopy is a powerful technique that combines the strengths of fluorescence microscopy and electron microscopy. The first enables rapid searching for regions of interest in large fields of view while the latter exhibits superior resolution over a narrow field of view. Routine use of correlative microscopy is seriously hampered by the cumbersome and elaborate experimental procedures. This is partly due to the use of two separate microscopes for fluorescence and electron microscopy. Here, an integrated approach to correlative microscopy is presented based on a laser scanning fluorescence microscope integrated in a transmission electron microscope. Using this approach the search for features in the specimen is greatly simplified and the time to carry out the experiment is strongly reduced. The potential of the integrated approach is demonstrated at room temperature on specimens of rat intestine cells labeled with AlexaFluor488 conjugated to wheat germ agglutinin and on rat liver peroxisomes immunolabeled with anti-catalase antibodies and secondary AlexaFluor488 antibodies and 10nm protein A-gold.
Keywords
Animals, Fluorescent Antibody Technique, Fluorescent Dyes, Intestines/cytology, Liver/ultrastructure, Microscopy, Confocal/instrumentation, Microscopy, Confocal/methods, Microscopy, Electron, Transmission/instrumentation, Microscopy, Electron, Transmission/methods, Microscopy, Fluorescence/instrumentation, Microscopy, Fluorescence/methods, Peroxisomes/ultrastructure, Rats
Pubmed
Web of science
Create date
28/02/2012 19:41
Last modification date
20/08/2019 17:21
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