A new low-molecular-weight component promoting adrenergic development in cultured chick sympathetic neurons

Détails

ID Serval
serval:BIB_F4354756DAC2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A new low-molecular-weight component promoting adrenergic development in cultured chick sympathetic neurons
Périodique
Journal of Neuroscience
Auteur(s)
Zurn  A. D.
ISSN
0270-6474 (Print)
Statut éditorial
Publié
Date de publication
11/1987
Volume
7
Numéro
11
Pages
3566-73
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
A low-molecular-weight component present in medium conditioned by cultured chick liver cells (LCM) enhances the adrenergic properties of dissociated chick superior cervical ganglion (SCG) neurons in culture (Zurn and Mudry, 1986). This substance cannot replace NGF as a survival, growth, or differentiation factor. However, in the presence of NGF, it stimulates neuronal metabolism and catecholamine (CA), but not ACh production by the SCG neurons. The effect on transmitter production is greater than that on neuronal metabolism. Yet this is not due to an increase in the specific activity of tyrosine hydroxylase (TH), the rate-limiting enzyme in CA synthesis. Interestingly, the effect of LCM on CA and ACh production, but not on neuronal metabolism, is potentiated in the presence of a large excess of NGF. The active component(s) present in LCM has a molecular weight lower than 500 Da and is not inactivated by heat or pronase treatment. So far, none of the small molecules tested (ascorbic acid, pyruvate, glucose, L-glutamic acid, glutathione, etc.) were able to mimic the effects of LCM on the SCG neurons. Thus this report describes a novel low-molecular-weight component different from NGF that promotes metabolism and adrenergic development in cultured chick sympathetic neurons.
Mots-clé
Acetylcholine/biosynthesis Animals Catecholamines/biosynthesis Cell Division/drug effects Cells, Cultured Chick Embryo Culture Media/analysis Ganglia, Sympathetic/*drug effects Growth Substances/*analysis/pharmacology L-Lactate Dehydrogenase/metabolism Liver Molecular Weight Nerve Growth Factors/pharmacology Neurons/*drug effects/metabolism Tyrosine 3-Monooxygenase/metabolism
Pubmed
Web of science
Création de la notice
28/01/2008 9:44
Dernière modification de la notice
03/03/2018 22:43
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