A new low-molecular-weight component promoting adrenergic development in cultured chick sympathetic neurons

Details

Serval ID
serval:BIB_F4354756DAC2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A new low-molecular-weight component promoting adrenergic development in cultured chick sympathetic neurons
Journal
Journal of Neuroscience
Author(s)
Zurn  A. D.
ISSN
0270-6474 (Print)
Publication state
Published
Issued date
11/1987
Volume
7
Number
11
Pages
3566-73
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
A low-molecular-weight component present in medium conditioned by cultured chick liver cells (LCM) enhances the adrenergic properties of dissociated chick superior cervical ganglion (SCG) neurons in culture (Zurn and Mudry, 1986). This substance cannot replace NGF as a survival, growth, or differentiation factor. However, in the presence of NGF, it stimulates neuronal metabolism and catecholamine (CA), but not ACh production by the SCG neurons. The effect on transmitter production is greater than that on neuronal metabolism. Yet this is not due to an increase in the specific activity of tyrosine hydroxylase (TH), the rate-limiting enzyme in CA synthesis. Interestingly, the effect of LCM on CA and ACh production, but not on neuronal metabolism, is potentiated in the presence of a large excess of NGF. The active component(s) present in LCM has a molecular weight lower than 500 Da and is not inactivated by heat or pronase treatment. So far, none of the small molecules tested (ascorbic acid, pyruvate, glucose, L-glutamic acid, glutathione, etc.) were able to mimic the effects of LCM on the SCG neurons. Thus this report describes a novel low-molecular-weight component different from NGF that promotes metabolism and adrenergic development in cultured chick sympathetic neurons.
Keywords
Acetylcholine/biosynthesis Animals Catecholamines/biosynthesis Cell Division/drug effects Cells, Cultured Chick Embryo Culture Media/analysis Ganglia, Sympathetic/*drug effects Growth Substances/*analysis/pharmacology L-Lactate Dehydrogenase/metabolism Liver Molecular Weight Nerve Growth Factors/pharmacology Neurons/*drug effects/metabolism Tyrosine 3-Monooxygenase/metabolism
Pubmed
Web of science
Create date
28/01/2008 8:44
Last modification date
20/08/2019 16:21
Usage data