Cutting edge: IL-23 receptor gfp reporter mice reveal distinct populations of IL-17-producing cells.

Détails

ID Serval
serval:BIB_F389524E2899
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cutting edge: IL-23 receptor gfp reporter mice reveal distinct populations of IL-17-producing cells.
Périodique
Journal of immunology
Auteur(s)
Awasthi A., Riol-Blanco L., Jäger A., Korn T., Pot C., Galileos G., Bettelli E., Kuchroo V.K., Oukka M.
ISSN
1550-6606 (Electronic)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
15/05/2009
Peer-reviewed
Oui
Volume
182
Numéro
10
Pages
5904-5908
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
IL-23, an IL-12 family member, has been implicated in the development of Th17 cells and the progression of autoimmune diseases. However, due to the lack of availability of sensitive Ab reagents specific for the IL-23 receptor (IL-23R), it has been difficult to characterize the cell types that express the IL-23R and are responsive to IL-23 in vivo. To address the role of IL-23 in vivo, we have generated a novel "knock-in" mouse in which we have replaced the intracellular domain of the IL-23R with the GFP. We show that in addition to Th17 cells, a subset of myeloid cells express IL-23R and respond to IL-23 by producing IL-17 and IL-22. Our studies further demonstrate that IL-23R expression is crucial for generation of encephalitogenic Th17 cells, but its expression on the innate immune system is dispensible in the development of experimental autoimmune encephalomyelitis.
Mots-clé
Animals, Encephalomyelitis, Autoimmune, Experimental/immunology, Encephalomyelitis, Autoimmune, Experimental/metabolism, Flow Cytometry, Gene Knock-In Techniques, Genes, Reporter, Green Fluorescent Proteins/genetics, Interleukin-17/biosynthesis, Interleukin-23/immunology, Interleukin-23/metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Receptors, Interleukin/immunology, Receptors, Interleukin/metabolism, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocyte Subsets/immunology, T-Lymphocyte Subsets/metabolism, T-Lymphocytes, Helper-Inducer/immunology, T-Lymphocytes, Helper-Inducer/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/08/2018 17:14
Dernière modification de la notice
09/05/2019 3:26
Données d'usage