Allorestricted T lymphocytes with a high avidity T-cell receptor towards NY-ESO-1 have potent anti-tumor activity.

Détails

ID Serval
serval:BIB_F2BF74B0AA31
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Allorestricted T lymphocytes with a high avidity T-cell receptor towards NY-ESO-1 have potent anti-tumor activity.
Périodique
International journal of cancer. Journal international du cancer
Auteur(s)
Krönig H., Hofer K., Conrad H., Guilaume P., Müller J., Schiemann M., Lennerz V., Cosma A., Peschel C., Busch D.H., Romero P., Bernhard H.
ISSN
1097-0215[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
125
Numéro
3
Pages
649-655
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The cancer-testis antigen NY-ESO-1 has been targeted as a tumor-associated antigen by immunotherapeutical strategies, such as cancer vaccines. The prerequisite for a T-cell-based therapy is the induction of T cells capable of recognizing the NY-ESO-1-expressing tumor cells. In this study, we generated human T lymphocytes directed against the immunodominant NY-ESO-1(157-165) epitope known to be naturally presented with HLA-A*0201. We succeeded to isolate autorestricted and allorestricted T lymphocytes with low, intermediate or high avidity TCRs against the NY-ESO-1 peptide. The avidity of the established CTL populations correlated with their capacity of lysing HLA-A2-positive, NY-ESO-1-expressing tumor cell lines derived from different origins, e.g. melanoma and myeloma. The allorestricted NY-ESO-1-specific T lymphocytes displayed TCRs with the highest avidity and best anti-tumor recognition activity. TCRs derived from allorestricted, NY-ESO-1-specific T cells may be useful reagents for redirecting primary T cells by TCR gene transfer and, therefore, may facilitate the development of adoptive transfer regimens based on TCR-transduced T cells for the treatment of NY-ESO-1-expressing hematological malignancies and solid tumors.
Mots-clé
Antibody-Dependent Cell Cytotoxicity, Antigen Presentation, Antigens, Neoplasm/immunology, Cytotoxicity Tests, Immunologic, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Genes, T-Cell Receptor, HLA-A Antigens/immunology, Humans, Membrane Proteins/immunology, Neoplasm Proteins/immunology, Peptide Fragments/immunology, Protein Multimerization, Receptors, Antigen, T-Cell/immunology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/01/2010 15:57
Dernière modification de la notice
09/05/2019 3:23
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