Molecular architecture of the rapidly metabolized 32-kilodalton protein of photosystem II. Indications for COOH-terminal processing of a chloroplast membrane polypeptide.

Détails

ID Serval
serval:BIB_F04AAA26E67F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular architecture of the rapidly metabolized 32-kilodalton protein of photosystem II. Indications for COOH-terminal processing of a chloroplast membrane polypeptide.
Périodique
Journal of Biological Chemistry
Auteur(s)
Marder J.B., Goloubinoff P., Edelman M.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
1984
Peer-reviewed
Oui
Volume
259
Numéro
6
Pages
3900-3908
Langue
anglais
Résumé
The molecular architecture of the rapidly metabolized 32-kilodalton chloroplast protein was investigated. Modified proteolytic techniques were applied to construct a cleavage map for this photosynthetic membrane component. The portion which anchors the protein to the membrane was shown to be rich in hydrophobic amino acid residues, while the surface-exposed portion was richer in polar residues. The amino to carboxy polarity of the map was established by comparing oligopeptide radiolabeling data with the polypeptide sequence decoded from the gene. This showed that the anchor domain contains the NH2 terminus, and the exposed domain the COOH terminus. The 32-kilodalton protein was previously demonstrated to derive from a 33.5-kilodalton precursor. We now show that the precursor molecule has a COOH-terminal oligopeptide extension. A model relating precursor cleavage to membrane integration is presented. COOH-terminal processing is proposed to ensure a correct sequence of events for assembly of the 32-kilodalton protein into the photosynthetic membrane.
Mots-clé
Amino Acid Sequence, Chloroplasts/metabolism, Electrophoresis, Polyacrylamide Gel, Genes, Molecular Weight, Papain, Peptide Fragments/analysis, Photosynthesis, Photosynthetic Reaction Center Complex Proteins, Photosystem II Protein Complex, Plant Proteins/genetics, Plant Proteins/isolation & purification, Plants/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 21:02
Dernière modification de la notice
03/03/2018 22:36
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