Whole-mount confocal immunofluorescence of mammalian CNS.

Détails

ID Serval
serval:BIB_EFB695DD1F7E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Whole-mount confocal immunofluorescence of mammalian CNS.
Périodique
Brain Research. Brain Research Protocols
Auteur(s)
Luque J.M., Morante-Oria J., Riederer B.M., Fairén A.
ISSN
1385-299X (Print)
ISSN-L
1385-299X
Statut éditorial
Publié
Date de publication
2001
Volume
6
Numéro
3
Pages
129-133
Langue
anglais
Résumé
Bright-field wholemount labeling techniques applied to the mammalian central nervous system (CNS) offer advantages over conventional methods based on sections since an immediate and three-dimensional view of the stained components is provided. It thereby becomes possible to survey and count large number of cells and fibers in their natural relationships. The ability of confocal laser scanning microscopy to visualize in one focal plane the fluorescence associated with multiple markers could be most valuable by the availability of reliable wholemount fluorescent techniques. Accordingly, based in our previously published bright-field wholemount protocols [Brain Res. Prot. 2 (1998) 165-173], we have devised an effective immmunofluorescence wholemount procedure. We show that reliable wholemount fluorescent staining can be obtained using isolated complete CNS aged up to rat embryonic day 17, with antibodies penetration in the millimeter range. Examples are shown of preparations in which colocalization can be observed in nerve cells of cytoskeletal and calcium-binding proteins.
Mots-clé
Animals, Antibodies, Brain Chemistry, Calcium-Binding Protein, Vitamin D-Dependent/analysis, Calcium-Binding Protein, Vitamin D-Dependent/immunology, Cerebral Cortex/chemistry, Female, Fetus/chemistry, Fluorescent Antibody Technique/methods, Mammals, Microscopy, Confocal/methods, Neurofilament Proteins/analysis, Neurofilament Proteins/immunology, Pregnancy, Rats, Rats, Wistar
Pubmed
Web of science
Création de la notice
24/01/2008 15:34
Dernière modification de la notice
03/03/2018 22:35
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