Article: article from journal or magazin.
Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro.
Journal of Bacteriology
Tandemly repeated insertion sequence IS21, located on a suicide plasmid, promoted replicon fusion with bacteriophage lambda in vitro in the presence of ATP. This reaction was catalyzed in a cell extract containing the 45-kDa IstA protein (cointegrase) and the 30-kDa IstB helper protein of IS21 after both proteins had been overproduced in Escherichia coli. Without IstB, replicon fusion was inefficient and did not produce the 4-bp target duplications typical of IS21.
Artificial Gene Fusion, Bacterial Proteins/physiology, Bacteriophage lambda/genetics, DNA Transposable Elements, Escherichia coli/genetics, Replicon, Tandem Repeat Sequences
Web of science
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