Matrix metalloproteinases correlate with alveolar-capillary permeability alteration in lung ischemia-reperfusion injury.

Details

Serval ID
serval:BIB_EA9FA9D05CC2
Type
Article: article from journal or magazin.
Collection
Publications
Title
Matrix metalloproteinases correlate with alveolar-capillary permeability alteration in lung ischemia-reperfusion injury.
Journal
Transplantation
Author(s)
Soccal P.M., Gasche Y., Pache J.C., Schneuwly O., Slosman D.O., Morel D.R., Spiliopoulos A., Suter P.M., Nicod L.P.
ISSN
0041-1337[print], 0041-1337[linking]
Publication state
Published
Issued date
2000
Volume
70
Number
7
Pages
998-1005
Language
english
Abstract
BACKGROUND: Matrix metalloproteinases (MMPs) are able to degrade the endothelial basal lamina and increase vascular permeability. METHODS: In a porcine model of isolated-reperfused lung, we studied the alveolar-capillary permeability and the zymographic expression of MMP-9 and MMP-2 in the bronchoalveolar lavage fluid of lungs submitted ex vivo to ischemia in three preservation solutions [modified Euro-Collins (EC), low-potassium-dextran, modified-blood]. Twenty-two pigs were randomly divided into three groups according to the preservation solution used. One lung of each pig was rapidly reperfused and analyzed (control lung) although the other lung was reperfused and analyzed after 8 hr of ischemia (ischemic lung). RESULTS: Alveolar-capillary permeability, evaluated by the transferrin leak index, was increased after 8 hr of ischemia compared with controls in the three groups, but was significantly higher in the modified EC group. In the EC group, after 8 hr of ischemia, both proMMP-9 and MMP-9 increased significantly (8.8- and 22-fold, respectively) compared with controls and this increase correlated with the transferrin leak index. Neither proMMP-9 nor MMP-9 increased with the other two preservation solutions. The MMP-2 increase after ischemia was smaller and was also restricted to the EC group. CONCLUSION: MMP expression is enhanced during lung ischemia-reperfusion, especially in the presence of EC and this phenomenon correlates with the alteration of alveolar-capillary permeability.
Keywords
Animals, Bronchoalveolar Lavage Fluid/chemistry, Bronchoalveolar Lavage Fluid/cytology, Capillary Permeability/drug effects, Cell Count, Immunohistochemistry, Indium Radioisotopes/diagnostic use, Lung/blood supply, Matrix Metalloproteinase 2/biosynthesis, Matrix Metalloproteinase 9/biosynthesis, Reperfusion Injury/enzymology, Swine, Tissue Distribution, Transferrin/metabolism
Pubmed
Web of science
Create date
19/02/2010 18:55
Last modification date
20/08/2019 16:13
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