Cytoplasmic activation of GAF, an IFN-gamma-regulated DNA-binding factor
Details
Serval ID
serval:BIB_EA0C2B8516BC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cytoplasmic activation of GAF, an IFN-gamma-regulated DNA-binding factor
Journal
EMBO Journal
ISSN
0261-4189
Publication state
Published
Issued date
04/1991
Peer-reviewed
Oui
Volume
10
Number
4
Pages
927-32
Notes
Journal Article --- Old month value: Apr
Abstract
We have investigated events following treatment of cells with interferon-gamma (IFN-gamma) that lead to the immediate transcriptional activation of an inducible gene. A gamma-interferon activation factor (GAF) was activated in the cytoplasm of human fibroblasts immediately after IFN-gamma treatment and bound to a newly identified target DNA sequence, the gamma-interferon activation site (GAS). The time course of activation of GAF was different in fibroblasts and HeLa cells and correlated well with IFN-gamma-induced transcriptional activation in both cell types. IFN-gamma-dependent activation of GAF also occurred in enucleated cells (cytoplasts), showing that an inactive cytoplasmic precursor is converted to the active factor. These findings support the concept that ligand-specific signals originating at the cell surface are transmitted through latent cytoplasmic proteins which are activated to bind specific DNA sites and then move to the nucleus to activate the transcription of specific sets of genes.
Keywords
Base Sequence
Cell Line
Cytochalasin B/pharmacology
DNA-Binding Proteins/*metabolism
Exodeoxyribonucleases
Fibroblasts/drug effects/metabolism
GTP-Binding Proteins/biosynthesis/*genetics
Gene Expression Regulation/drug effects
Hela Cells/drug effects/metabolism
Humans
Interferon Type II/*metabolism
Interferon-gamma, Recombinant/*pharmacology
Kinetics
Molecular Sequence Data
Plasmids
Promoter Regions (Genetics)
*Transcription, Genetic/drug effects
Pubmed
Web of science
Create date
25/01/2008 9:41
Last modification date
20/08/2019 17:12