MDM2 and CDK4 immunostainings are useful adjuncts in diagnosing well-differentiated and dedifferentiated liposarcoma subtypes: a comparative analysis of 559 soft tissue neoplasms with genetic data

Details

Serval ID
serval:BIB_E9990FFD03B5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
MDM2 and CDK4 immunostainings are useful adjuncts in diagnosing well-differentiated and dedifferentiated liposarcoma subtypes: a comparative analysis of 559 soft tissue neoplasms with genetic data
Journal
American Journal of Surgical Pathology
Author(s)
Binh  M. B., Sastre-Garau  X., Guillou  L., de  P. G., Terrier  P., Lagace  R., Aurias  A., Hostein  I., Coindre  J. M.
ISSN
0147-5185 (Print)
Publication state
Published
Issued date
2005
Volume
29
Number
10
Pages
1340-1347
Notes
PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
Atypical lipomatous tumor/well-differentiated liposarcoma (ALT-WDLPS) and dedifferentiated liposarcoma (DDLPS) may be difficult to distinguish from benign adipose tumors and from poorly differentiated sarcomas, respectively. Genetically, they are characterized by amplification of MDM2 and CDK4 genes on chromosome 12q13-15. We examined a series of 559 soft tissue tumors (44 ALT-WDLPS, 61 DDLPS, 49 benign adipose tumors, and 405 non-ALT-WDLPS/DDLPS sarcomas) for MDM2 and CDK4 expression using immunohistochemistry. MDM2 and CDK4 immunoexpressions were compared with gene amplification status (as assessed by quantitative PCR and/or comparative genomic hybridization) in 241 neoplasms. Most ALT-WDLPS/DDLPS expressed MDM2 (97%) and CDK4 (92%) as opposed to few benign adipose tumors (MDM2, 5%; CDK4, 2%) and a limited number of non-ALT-WDLSP/DDLPS sarcomas (MDM2, 19%; CDK4, 6%). The sensitivity and specificity of MDM2 and CDK4 immunostainings in identifying ALT-WDLPS/DDLPS among other soft tissue tumors were 97% and 92%, and 83% and 95%, respectively. MDM2 and CDK4 immunostainings were particularly useful to separate ALT-WDLPS from the large group of differentiated adipose tumors, and to distinguish DDLPS from poorly differentiated sarcomas. A strong correlation was observed between MDM2 and CDK4 stainings and gene amplification status. In conclusion, MDM2 and CDK4 immunostainings, which correlate with gene amplification, are helpful adjuncts to differentiate ALT-WDLPS from benign adipose tumors and to separate DDLPS from poorly differentiated sarcomas
Keywords
Aged/Cyclin-Dependent Kinase 4/metabolism/Diagnosis,Differential/Gene Amplification/Humans/Immunohistochemistry/Liposarcoma/diagnosis/genetics/Male/Middle Aged/Polymerase Chain Reaction/Proto-Oncogene Proteins c-mdm2/Sensitivity and Specificity/Soft Tissue Neoplasms
Pubmed
Web of science
Create date
29/01/2008 18:35
Last modification date
20/08/2019 16:12
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