Inactivation of E alpha and E beta expression in inbred and wild mice by multiple distinct mutations, some of which predate speciation within Mus species.

Détails

ID Serval
serval:BIB_E6EE933E9954
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inactivation of E alpha and E beta expression in inbred and wild mice by multiple distinct mutations, some of which predate speciation within Mus species.
Périodique
International Immunology
Auteur(s)
Tacchini-Cottier F., Mayer W.E., Begovich A.B., Jones P.P.
ISSN
0953-8178 (Print)
ISSN-L
0953-8178
Statut éditorial
Publié
Date de publication
1995
Volume
7
Numéro
9
Pages
1459-1471
Langue
anglais
Résumé
The H-2 MHC of mice encodes two functional class II heterodimeric proteins: A alpha A beta (A) and E alpha E beta (E). While failure to express the A protein has not been reported, a significant proportion of of H-2 haplotypes in both inbred and wild mice do not express E proteins. We and others have previously characterized the molecular basis for defective E expression in haplotypes from Mus domesticus (b, f, q, s, from inbred strains) and M. castaneus (w17, wild-derived) species, identifying six distinct defects in the genes for E alpha or E beta. In this report we have extended these studies to other E- haplotypes, including several from t-haplotype-bearing M. domesticus mice (w29, w57, w302) and one derived from the Asian species M. bactrianus (w301). Analyses at the protein, RNA and DNA levels were employed to identify the defects in the genes for Ea and Eb. At least one new defect was identified that prevents E beta expression in a t-associated H-2 haplotypes (w57), bringing the number of distinct mutations causing the E- phenotype to seven. Another t-associated haplotype, w302, was found to share the same E beta defect with mice of the inbred q haplotype and of the w17 haplotype from M. castaneus, while its Ea gene contains the deletion carried also by the inbred b and s haplotypes and by a number of wild haplotypes. The mutations in the Ea and Eb genes of the w301 haplotype from M. bactrianus were found to be identical to those of the inbred f haplotype. This indicates that the origin of the mutations in the Eb genes of the q, w17 and w302 haplotypes and in the Ea and Eb genes of the f and w301 haplotypes, predated speciation within Mus, thought to have occurred approximately 0.35-1 million years ago. Their maintenance in mouse populations suggests that in certain conditions the failure to express E alpha E beta proteins may be advantageous and selected for.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Blotting, Southern, Genes, MHC Class II, H-2 Antigens/genetics, Haplotypes, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Sequence Data, Muridae/immunology, Mutation, RNA/analysis
Pubmed
Web of science
Création de la notice
24/01/2008 16:09
Dernière modification de la notice
20/08/2019 17:10
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