Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF).
Details
Serval ID
serval:BIB_E6E038881A19
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF).
Journal
Journal of Cell Biology
ISSN
0021-9525 (Print)
ISSN-L
0021-9525
Publication state
Published
Issued date
1997
Peer-reviewed
Oui
Volume
136
Number
2
Pages
307-317
Language
english
Abstract
Vacuole inheritance in yeast involves the formation of tubular and vesicular "segregation structures" which migrate into the bud and fuse there to establish the daughter cell vacuole. Vacuole fusion has been reconstituted in vitro and may be used as a model for an NSF-dependent reaction of priming, docking, and fusion. We have developed biochemical and microscopic assays for the docking step of in vitro vacuole fusion and characterized its requirements. The vacuoles must be primed for docking by the action of Sec17p (alpha-SNAP) and Sec18p (NSF). Priming is necessary for both fusion partners. It produces a labile state which requires rapid docking in order to lead productively to fusion. In addition to Sec17p/Sec18p, docking requires the activity of the Ras-like GTPase Ypt7p. Unlike Sec17p/Sec18p, which must act before docking, Ypt7p is directly involved in the docking process itself.
Keywords
Adenosine Triphosphatases, Adenosine Triphosphate/metabolism, Carrier Proteins/metabolism, Cell Fractionation, Cytosol/metabolism, Dimerization, Fungal Proteins/metabolism, GTP Phosphohydrolases/metabolism, GTP-Binding Proteins/metabolism, Kinetics, Membrane Fusion, Membrane Proteins/metabolism, Microscopy, Mutation, Repressor Proteins, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae/ultrastructure, Saccharomyces cerevisiae Proteins, Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins, Temperature, Thioredoxins/metabolism, Vacuoles/metabolism, Vesicular Transport Proteins, rab GTP-Binding Proteins
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 16:06
Last modification date
20/08/2019 17:09
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