Streptozotocin induces a shift towards T regulatory cell responses in vivo

Details

Serval ID
serval:BIB_E61A4545288D
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Streptozotocin induces a shift towards T regulatory cell responses in vivo
Title of the conference
45th Congress of the European Society for Surgical Research
Author(s)
Muller Y. D., Ehirchiou D., Morel P., Avril I., Serre-Beinier V., Yung G. Puga , Golshayan D., Buehler L. H., Seebach J. D.
Address
Geneva - SWITZERLAND, 9-12 June, 2010
ISBN
0007-1323
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
97
Series
British Journal of Surgery
Pages
S55
Language
english
Notes
Meeting Abstract
Abstract
Objectives:
Streptozotocin (STZ) induced diabetes is currently the most commonly used animalmodel for islet transplantation.However, STZtreatment and the ensuing hyperglycemia were both shown to affect the immune response, including an apparent induction of lymphopenia. The aim of this study was to evaluate the respective effect of STZ and hyperglycemia on the immune system in STZ induced diabetic C57BL/6 mice.
Methods:
Phenotypes and levels of T and B cells were analyzed by flow cytometry in blood and spleen over time. The effect of hyperglycemia was further characterized by insulin replacement, islet transplantation and by using Rip (rat insulin promoter) DTR (dipheteria tocin receptor) transgenic mice.
Results:
STZ but not hyperglycemia was toxic for splenocytes in vitro, whereas hyperglycemia correlated with diabetes associated blood and spleen lymphopenia in vivo. Moreover, independently of hyperglycemia, STZ lead to a relative increase of T regulatory cells which retained their suppressive capacity in vitro.
Conclusion:
These data suggest thatSTZand the ensuing acute hyperglycemia have major direct and indirect effects on immune homeostasis. Thus, high caution needs to be exercised in the interpretation of the results of tolerance induction and/or immunosuppressive protocols in STZ-induced diabetes and islet transplantation models.
Web of science
Create date
21/10/2010 10:15
Last modification date
20/08/2019 16:09
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