Production and characterization of four anti-neuropeptide Y monoclonal antibodies
Details
Serval ID
serval:BIB_E31065477959
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Production and characterization of four anti-neuropeptide Y monoclonal antibodies
Journal
Hybridoma
ISSN
0272-457X (Print)
Publication state
Published
Issued date
08/1992
Volume
11
Number
4
Pages
409-24
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
Human neuropeptide Y (hNPY) is a potent vasoconstrictor peptide of 36 aminoacid residues. We isolated hybridomas secreting four monoclonal antibodies directed against various epitopes of neuropeptide Y and studied their cross-reactivity with peptide YY (PYY) and the pancreatic polypeptide (PP), two peptides sharing sequence homologies with hNPY (respectively 70% and 50%). The antibody NPY02 is an IgG1 with a Ka of 5.5 x 10(10) liters/mol. It binds to the 11-24 region of NPY (IC50 = 2 x 10(-7)M), does not recognize PP but cross-reacts weakly with PYY. Antibodies NPY03 and NPY05 are IgG2 with respective Ka's of 6.7 x 10(9) and 2.5 x 10(10) liters/mol. They interact with a C-terminal epitope on NPY (NPY 27-34, IC50 = 2 x 10(-9) M for NPY03 and NPY 32-36, IC50 = 1 x 10(-9) M for NPY05). These two antibodies cross-react with PYY whereas only NPY05 binds PP. NPY05 is unable to bind the free acid form of neuropeptide Y. The 32-36 COOH free subpeptide is recognized 50,000 less efficiently by NPY05 than its amidated form. Antibody NPY04 is an IgG3 with a Ka of 3.8 x 10(8) liters/mol. It recognizes a N-terminal epitope between aminoacids 1 and 12 (IC50 = 2.5 x 10(-6) M). NPY04 interacts weakly with PYY but not detectably with PP. These results obtained with 4 different monoclonal antibodies demonstrate the presence of at least four epitopes on hNPY, two of them being continuous. These antibodies will be used to study the interaction of NPY with its receptor and to develop sensitive and specific assays for determination of NPY concentrations in biological fluids.
Keywords
Amino Acid Sequence
Animals
Antibodies, Monoclonal/*biosynthesis/isolation & purification
Cross Reactions
Epitopes/chemistry
Humans
Hybridomas/immunology
Immunoradiometric Assay
Mice
Molecular Sequence Data
Neuropeptide Y/analysis/chemistry/*immunology
Pancreatic Polypeptide/chemistry/immunology
Peptide YY
Peptides/chemistry/immunology
Pubmed
Web of science
Create date
25/01/2008 11:55
Last modification date
20/08/2019 17:06