Role of the cystic fibrosis transmembrane conductance regulator in internalization of Pseudomonas aeruginosa by polarized respiratory epithelial cells

Details

Serval ID
serval:BIB_E1BEA06B8F3C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Role of the cystic fibrosis transmembrane conductance regulator in internalization of Pseudomonas aeruginosa by polarized respiratory epithelial cells
Journal
Cell Microbiol
Author(s)
Darling K. E., Dewar A., Evans T. J.
ISSN
1462-5814 (Print)
ISSN-L
1462-5814
Publication state
Published
Issued date
06/2004
Volume
6
Number
6
Pages
521-33
Language
english
Notes
Darling, Katharine E A
Dewar, Ann
Evans, Thomas J
eng
Research Support, Non-U.S. Gov't
England
Cell Microbiol. 2004 Jun;6(6):521-33. doi: 10.1111/j.1462-5822.2004.00380.x.
Abstract
Pseudomonas aeruginosa is an important human pathogen, producing lung infection in individuals with cystic fibrosis (CF), patients who are ventilated and those who are neutropenic. The respiratory epithelium provides the initial barrier to infection. Pseudomonas aeruginosa can enter epithelial cells, although the mechanism of entry and the role of intracellular organisms in its life cycle are unclear. We devised a model of infection of polarized human respiratory epithelial cells with P. aeruginosa and investigated the role of the cystic fibrosis transmembrane conductance regulator (CFTR) in adherence, uptake and IL-8 production by human respiratory epithelial cells. We found that a number of P. aeruginosa strains could invade and replicate within cells derived from a patient with CF. Intracellular bacteria did not produce host cell cytotoxicity over a period of 24 h. When these cells were transfected with wild-type CFTR, uptake of bacteria was significantly reduced and release of IL-8 following infection enhanced. We propose that internalized P. aeruginosa may play an important role in the pathogenesis of infection and that, by allowing greater internalization into epithelial cells, mutant CFTR results in an increased susceptibility of bronchial infection with this microbe.
Keywords
Apoptosis, Bacterial Adhesion, Cell Line, Cell Polarity, Colony Count, Microbial, Cystic Fibrosis/microbiology, Cystic Fibrosis Transmembrane Conductance Regulator/*genetics/*metabolism, Cytoplasm/microbiology, Epithelial Cells/*microbiology/ultrastructure, Gene Deletion, Humans, Interleukin-8/analysis/biosynthesis, Microscopy, Confocal, Microscopy, Electron, Mutation, Pseudomonas aeruginosa/growth & development/*physiology, Respiratory Mucosa/*microbiology/ultrastructure
Pubmed
Create date
07/04/2020 14:20
Last modification date
08/04/2020 5:26
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