Recognition of a B cell lymphoma by anti-idiotypic T cells.

Détails

ID Serval
serval:BIB_DFB7D67D386C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Recognition of a B cell lymphoma by anti-idiotypic T cells.
Périodique
Journal of Immunology
Auteur(s)
Wilson A., George A.J., King C.A., Stevenson F.K.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
1990
Volume
145
Numéro
11
Pages
3937-3943
Langue
anglais
Résumé
Idiotypic IgM derived from a B cell lymphoma can act as a tumor-associated Ag, in that immunization with this purified protein generates an anti-idiotypic immune response that specifically suppresses tumor development. Spleens of immune mice contain T cells that proliferate in response to idiotypic IgM. However, idiotypic Ag is presented to the T cells most efficiently in its natural form at the surface of the lymphoma cells, than as soluble IgM plus presenting cells. Variant tumors that display either little or no idiotypic IgM at the cell surface, but which are otherwise indistinguishable from parental tumor, induce a weak response or fail to stimulate the T cells, respectively. Anti-idiotypic lines and clones have been derived from the splenic T cells by growth in the presence of irradiated tumor cells. Phenotypic analysis revealed that cells from both lines and clones express CD3 and CD4 Ag, but not CD8. Recognition of tumor Id, which required no added presenting cells, was inhibited by antibody against MHC class II Ag, and variably by anti-CD4. Proliferative responses were inhibited by anti-idiotypic antibodies, but also by antibodies against the constant region of the mu H chain, indicating that perturbation of the surface IgM abrogates availability of idiotypic determinants to the T cells.
Mots-clé
Animals, Antibodies, Anti-Idiotypic/immunology, Clone Cells, Immunoglobulin M/immunology, Lymphocyte Activation, Lymphoma, B-Cell/immunology, Mice, Mice, Inbred BALB C, T-Lymphocytes/immunology, Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
11/04/2013 10:39
Dernière modification de la notice
20/08/2019 16:04
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