Macro and microheterogeneity in normal endothelial cells: differential composition of luminal glycocalyx and functional implications.

Details

Serval ID
serval:BIB_DF4AFC26BFAB
Type
Article: article from journal or magazin.
Collection
Publications
Title
Macro and microheterogeneity in normal endothelial cells: differential composition of luminal glycocalyx and functional implications.
Journal
Endothelium : Journal of Endothelial Cell Research
Author(s)
Brouland J.P., Gilbert M.A., Bonneau M., Pignaud G., Bal Dit Solier C., Drouet L.
ISSN
1062-3329 (Print)
ISSN-L
1026-793X
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
6
Number
3
Pages
251-262
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
Endothelial cells (EC) are involved in various physiological and pathological processes through the expression of their surface glycoproteins. They are covered by the glycocalyx, composed of glucidic residues from cell surface membrane glycoproteins, glycoplipids and proteoglycans. Glucidic sequences can be specifically characterized by their binding to lectins. Eight lectins were used to investigate the distribution and regulation of EC surface glucidic residues in various blood vessels of adult and newborn pigs. EC lectin binding was compared to von Willebrand factor (vWF) expression as EC reference marker. Six out of eight lectins (BSI-B4, DBA, EEA, HP, MAL I and PNA) were helpful for this determination. Considering only the intensity of labelings, vWF and DBA gave the best stainings of adult pig ECs. In newborn pigs, the best labelings were obtained with EEA and MAL I. Furthermore, the distribution of lectin binding to ECs and EC vWF expression was heterogeneous depending on the EC location along vascular tree and age. Beside this macroheterogeneity this study highlights a microheterogeneity of EC lectin binding and vWF expression in situ, defined as a staining of equal intensity by individual ECs, scattered among negative ones, in a given vascular segment. EC surface sugar residues were differently modulated in newborn and adult pig ECs and differently according to EC vWF expression. The functional involvement of EC glycocalyx was reflected by EC lectin binding in the spleen and liver. This study emphasizes the high level of EC heterogeneity for various markers. The EC macro- and microheterogeneity reflect the "plasticity" or "unstability" of EC phenotypes and suggests that ECs are subject to several levels of regulation and are probably grouped in functional clusters to best adjust their functions to microenvironmental requirements. This concept must be considered in further investigations notably in in vitro studies where EC phenotype can be altered.
Keywords
Animals, Animals, Newborn, Biological Markers, Endothelium, Vascular/chemistry, Endothelium, Vascular/cytology, Glycocalyx/chemistry, Glycocalyx/physiology, Swine, von Willebrand Factor/metabolism
Pubmed
Web of science
Create date
13/10/2015 9:09
Last modification date
20/08/2019 16:03
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