Hunchback-independent silencing of late Ubx enhancers by a Polycomb Group Response Element.

Details

Ressource 1Download: BIB_DDEC1BD92884.P001.pdf (3042.87 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_DDEC1BD92884
Type
Article: article from journal or magazin.
Collection
Publications
Title
Hunchback-independent silencing of late Ubx enhancers by a Polycomb Group Response Element.
Journal
Embo Journal
Author(s)
Poux S., Kostic C., Pirrotta V.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
15
Number
17
Pages
4713-4722
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
Drosophila homeotic genes are kept silent outside of their appropriate expression domains by a repressive chromatin complex formed by the Polycomb Group proteins. In the case of the Ubx gene, it has been proposed that the early repressor HB, binding at enhancers, recruits the Polycomb complex and specifies the domain of repression. We show that some Ubx enhancers are activated after blastoderm. If a Polycomb Response Element (PRE) is combined with such late enhancers, repression of a reporter gene can be established everywhere in the embryo, irrespective of the presence or absence of hunchback protein. If, however, these late enhancers are combined with a Ubx early enhancer, as well as a PRE, repression is established only where the reporter gene was inactive at early stages. These results imply that the Polycomb complex is not dependent on hunchback and suggest that the pattern of silencing reflects rather the state of activity of the gene at the time the Polycomb complex is formed.
Keywords
Animals, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Drosophila/embryology, Drosophila/genetics, Drosophila Proteins, Enhancer Elements, Genetic, Genes, Homeobox, Homeodomain Proteins/genetics, Transcription Factors/metabolism
Pubmed
Create date
29/09/2014 9:44
Last modification date
20/08/2019 16:02
Usage data