Cell-surface expression of the channel activating protease xCAP-1 is required for activation of ENaC in the Xenopus oocyte

Details

Serval ID
serval:BIB_DC25FB42EFE8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cell-surface expression of the channel activating protease xCAP-1 is required for activation of ENaC in the Xenopus oocyte
Journal
Journal of the American Society of Nephrology
Author(s)
Vallet  V., Pfister  C., Loffing  J., Rossier  B. C.
ISSN
1046-6673
Publication state
Published
Issued date
03/2002
Peer-reviewed
Oui
Volume
13
Number
3
Pages
588-94
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
Sodium balance, extracellular fluid volume, and ultimately BP are maintained by precise regulation of the activity of the epithelial sodium channel (ENaC). Using a functional complementation assay in the Xenopus laevis oocyte expression system, a channel-activating protease (CAP-1) that increases ENaC activity two to threefold in the Xenopus oocyte expression system is here identified. External application of trypsin mimics the effect of Xenopus CAP-1 (xCAP-1) on ENaC activity, which can be blocked by aprotinin, a serine protease inhibitor, suggesting the existence of a novel extracellular pathway for controlling ENaC activity. Sequence analysis predicts that CAP-1 is a secreted and/or glycosyl-phosphatidyl-inositol (GPI)-anchored protein. The aim of the present study was to determine whether cell-surface expression of xCAP-1 is required for ENaC activation. By site-directed mutagenesis of xCAP-1, the importance of the catalytic site, N-glycosylation, and the GPI anchor of xCAP-1 on ENaC activity were analyzed. Glycosylation or catalytic activity is not required for cell-surface expression of xCAP-1, whereas the deletion of the GPI anchor consensus motif at the C-terminus of xCAP-1 (G305Stop) abolishes cell-surface expression and ENaC activation. G305Stop-mutated xCAP-1 is recovered as a secreted protein in the external medium. A catalytic mutant of xCAP-1 significantly decreased ENaC activation but did not fully abolish the effect of xCAP-1. The data indicate the critical role of the GPI anchor in ENaC activation and suggest that catalytic and noncatalytic mechanisms are involved.
Keywords
Amino Acid Sequence/genetics Animals Cell Membrane/metabolism Epithelial Sodium Channel Female Glycosylation Glycosylphosphatidylinositols/physiology Oocytes/*metabolism Serine Endopeptidases/genetics/*metabolism Sodium Channels/*physiology Xenopus laevis
Pubmed
Web of science
Create date
24/01/2008 13:00
Last modification date
20/08/2019 16:01
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