Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_D98515AFF191
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules.
Périodique
Nucleic Acids Research
Auteur(s)
Cebrián J., Kadomatsu-Hermosa M.J., Castán A., Martínez V., Parra C., Fernández-Nestosa M.J., Schaerer C., Martínez-Robles M.L., Hernández P., Krimer D.B., Stasiak A., Schvartzman J.B.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Statut éditorial
Publié
Date de publication
2015
Volume
43
Numéro
4
Pages
e24
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
We systematically varied conditions of two-dimensional (2D) agarose gel electrophoresis to optimize separation of DNA topoisomers that differ either by the extent of knotting, the extent of catenation or the extent of supercoiling. To this aim we compared electrophoretic behavior of three different families of DNA topoisomers: (i) supercoiled DNA molecules, where supercoiling covered the range extending from covalently closed relaxed up to naturally supercoiled DNA molecules; (ii) postreplicative catenanes with catenation number increasing from 1 to ∼15, where both catenated rings were nicked; (iii) knotted but nicked DNA molecules with a naturally arising spectrum of knots. For better comparison, we studied topoisomer families where each member had the same total molecular mass. For knotted and supercoiled molecules, we analyzed dimeric plasmids whereas catenanes were composed of monomeric forms of the same plasmid. We observed that catenated, knotted and supercoiled families of topoisomers showed different reactions to changes of agarose concentration and voltage during electrophoresis. These differences permitted us to optimize conditions for their separation and shed light on physical characteristics of these different types of DNA topoisomers during electrophoresis.
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/04/2015 7:14
Dernière modification de la notice
20/08/2019 15:58
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