Modulatory effects of acid-sensing ion channels on action potential generation in hippocampal neurons.

Détails

ID Serval
serval:BIB_D92E31799070
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Modulatory effects of acid-sensing ion channels on action potential generation in hippocampal neurons.
Périodique
American Journal of Physiology. Cell Physiology
Auteur(s)
Vukicevic M., Kellenberger S.
ISSN
0363-6143[print], 0363-6143[linking]
Statut éditorial
Publié
Date de publication
2004
Volume
287
Numéro
3
Pages
C682-C690
Langue
anglais
Résumé
Extracellular acidification has been shown to generate action potentials (APs) in several types of neurons. In this study, we investigated the role of acid-sensing ion channels (ASICs) in acid-induced AP generation in brain neurons. ASICs are neuronal Na(+) channels that belong to the epithelial Na(+) channel/degenerin family and are transiently activated by a rapid drop in extracellular pH. We compared the pharmacological and biophysical properties of acid-induced AP generation with those of ASIC currents in cultured hippocampal neurons. Our results show that acid-induced AP generation in these neurons is essentially due to ASIC activation. We demonstrate for the first time that the probability of inducing APs correlates with current entry through ASICs. We also show that ASIC activation in combination with other excitatory stimuli can either facilitate AP generation or inhibit AP bursts, depending on the conditions. ASIC-mediated generation and modulation of APs can be induced by extracellular pH changes from 7.4 to slightly <7. Such local extracellular pH values may be reached by pH fluctuations due to normal neuronal activity. Furthermore, in the plasma membrane, ASICs are localized in close proximity to voltage-gated Na(+) and K(+) channels, providing the conditions necessary for the transduction of local pH changes into electrical signals.
Mots-clé
Acids, Action Potentials/physiology, Animals, Cells, Cultured, Electrophysiology, Embryo, Mammalian, Extracellular Fluid/chemistry, Hippocampus/physiology, Hydrogen-Ion Concentration, Ion Channels/physiology, Mice, Neurons/physiology, Patch-Clamp Techniques, Recombinant Proteins/metabolism, Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 13:45
Dernière modification de la notice
03/03/2018 21:52
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