Mate attraction is widespread among animals and appears to facilitate mating and to prevent hybridisation between closely related species. In this study we investigated mate preference between two geographical isolates of Echinostoma caproni (Trematoda, Platyhelminth) and another species of the genus Echinostoma E. sp. Because previous experiments showed a partial reproductive isolation between echinostome isolates, we examined the possibility that such isolation resulted from differential mate attraction. We compared intra-isolate, inter-isolate and interspecific pairings using two in vitro experimental designs. In the first experiment we compared mate attraction of two individuals belonging to or not belonging to the same isolate, while in the second experiment we examined mate choice when individuals were in the presence of individuals from both the same isolate and from a different isolate or a different species. Distances between worms were measured over a period of 90 min. Results from both experiments suggested that mate attraction was similar for intra-isolate, inter-isolate or interspecific combinations. This lack of mate preference in vitro would therefore support an alternative hypothesis of a reproductive isolation through sperm selection.