Combining Standard Molecular Typing and Whole Genome Sequencing to Investigate Pseudomonas aeruginosa Epidemiology in Intensive Care Units.

Details

Ressource 1Download: Magalhães_2020_FrontPublicHealth_Combining Standard Molecular Typing and WGSg to Investigate P aeruginosa Epidemiology in ICU.pdf (2042.70 [Ko])
State: Public
Version: author
License: CC BY 4.0
Serval ID
serval:BIB_D83F2D6A3BC0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Combining Standard Molecular Typing and Whole Genome Sequencing to Investigate Pseudomonas aeruginosa Epidemiology in Intensive Care Units.
Journal
Frontiers in public health
Author(s)
Magalhães B., Valot B., Abdelbary MMH, Prod'hom G., Greub G., Senn L., Blanc D.S.
ISSN
2296-2565 (Print)
ISSN-L
2296-2565
Publication state
Published
Issued date
2020
Peer-reviewed
Oui
Volume
8
Pages
3
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Pseudomonas aeruginosa is one of the main pathogens responsible for nosocomial infections, particularly in Intensive Care Units (ICUs). Due to the complexity of P. aeruginosa ecology, only powerful typing methods can efficiently allow its surveillance and the detection during expanding outbreaks. An increase in P. aeruginosa incidence was observed in the ICUs of the Lausanne University Hospital between 2010 and 2014. All clinical and environmental isolates retrieved during this period were typed with Double locus sequence typing (DLST), which detected the presence of three major genotypes: DLST 1-18, DLST 1-21, and DLST 6-7. DLST 1-18 (ST1076) isolates were previously associated with an epidemiologically well-described outbreak in the burn unit. Nevertheless, DLST 1-21 (ST253) and DLST 6-7 (ST17) showed sporadic occurrence with only few cases of possible transmission between patients. Whole genome sequencing (WGS) was used to further investigate the epidemiology of these three major P. aeruginosa genotypes in the ICUs. WGS was able to differentiate between outbreak and non-outbreak isolates and confirm suspected epidemiological links. Additionally, whole-genome single nucleotide polymorphisms (SNPs) results considered isolates as closely related for which no epidemiological links were suspected, expanding the epidemiological investigation to unsuspected links. The combination of a first-line molecular typing tool (DLST) with a more discriminatory method (WGS) proved to be an accurate and cost-efficient typing strategy for the investigation of P. aeruginosa epidemiology in the ICUs.
Keywords
Pseudomonas aeruginosa, double locus sequence typing, genomic epidemiology, molecular epidemiology, molecular typing, whole genome sequencing
Pubmed
Open Access
Yes
Create date
14/02/2020 16:31
Last modification date
21/02/2020 7:10
Usage data