CD44 as a receptor for colonization of the pharynx by group A Streptococcus.

Details

Serval ID
serval:BIB_D7323721633E
Type
Article: article from journal or magazin.
Collection
Publications
Title
CD44 as a receptor for colonization of the pharynx by group A Streptococcus.
Journal
Journal of Clinical Investigation
Author(s)
Cywes C., Stamenkovic I., Wessels M.R.
ISSN
0021-9738[print], 0021-9738[linking]
Publication state
Published
Issued date
2000
Volume
106
Number
8
Pages
995-1002
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
The pharynx is the primary reservoir for strains of group A STREPTOCOCCUS: (GAS) associated both with pharyngitis (streptococcal sore throat) and with invasive or "flesh-eating" soft tissue infections. We now report that CD44, a hyaluronic acid-binding protein that mediates human cell-cell- and cell-extracellular matrix-binding interactions, functions as a receptor for GAS colonization of the pharynx in vivo. We found that attachment of GAS to murine epithelial keratinocytes was mediated by binding of the GAS hyaluronic acid capsular polysaccharide to CD44. In studies of transgenic mice with a selective defect in epithelial expression of CD44, GAS adherence to CD44-deficient keratinocytes in vitro was reduced compared with adherence to keratinocytes expressing normal levels of CD44. After intranasal inoculation, GAS colonized the oropharynx of wild-type mice but failed to colonize transgenic mice deficient in CD44 expression. GAS colonization of wild-type mice could be blocked by coadministration of mAb to CD44 or by pretreatment of the animals with exogenous hyaluronic acid. These results provide evidence that CD44 serves as a receptor for GAS colonization of the pharynx and support the potential efficacy of disrupting the interaction between the GAS hyaluronic acid capsule and CD44 as a novel approach to preventing pharyngeal infection.
Keywords
Animals, Antigens, CD44/genetics, Antigens, CD44/metabolism, Bacterial Adhesion/drug effects, Bacterial Capsules/metabolism, Hyaluronic Acid/pharmacology, Keratinocytes/microbiology, Mice, Mice, Inbred C57BL, Mice, Transgenic, Pharynx/microbiology, Streptococcus pyogenes/pathogenicity
Pubmed
Open Access
Yes
Create date
26/08/2010 16:38
Last modification date
20/08/2019 15:56
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