Article: article from journal or magazin.
gp33-38, an early human T cell activation antigen
Journal of Immunology
Journal Article --- Old month value: Mar 15
We describe an activation Ag Me14/D12 that appears early after T cell activation and is absent in resting T lymphocytes. Me14/D12 is a nondisulfide-linked heterodimeric structure containing two polypeptide chains of 33,000 and 38,000 Da. The expression of Me14/D12 on resting T lymphocytes can be induced by different activation stimuli such as the lectins PHA and Con A, the phorbol ester PMA, and anti-CD3 mAb. The induction of mRNA for Me14/D12 (gp33-38) in PHA-activated T lymphocytes precedes that of IL-2R gene transcripts by more than 20 h. Me14/D12 mRNA was detectable as early as 2 h after the onset of activation and mRNA for the IL-2R only after 24 h. The surface expression of Me14/D12 was detectable between 12 and 24 h after activation and was maximal between 24 and 48 h. Several T leukemia cell lines express the Me14/D12 Ag. On Me14/D12- cell lines, PMA and IFN-gamma induced surface expression of Me14/D12. Once Me14/D12 Ag were expressed on Jurkat cells after stimulation with either PMA or IFN-gamma, the binding of mAb Me14/D12 induced the production of significant amounts of IL-2 and of Ca2+ mobilization from internal stores. Comparative biochemical studies clearly demonstrate that Me14/D12 (gp33-38) is different from the CD69 molecular complex defined by mAb MLR3 and AIM.
Antibodies, Monoclonal/immunology Antigens, CD3 Antigens, Differentiation, T-Lymphocyte/immunology Cell Line Hematopoietic Stem Cells/immunology Humans Interferon Type II/pharmacology Isoelectric Point Leukemia, T-Cell/immunology *Lymphocyte Activation Membrane Glycoproteins/*immunology Molecular Weight Phytohemagglutinins/pharmacology Precipitin Tests Receptors, Antigen, T-Cell/immunology T-Lymphocytes/*immunology Tetradecanoylphorbol Acetate/pharmacology Time Factors Tumor Cells, Cultured
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