T cell receptor delta gene rearrangement and T early alpha (TEA) expression in immature alpha beta lineage thymocytes: implications for alpha beta/gamma delta lineage commitment.

Détails

ID Serval
serval:BIB_D586EAFD0683
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
T cell receptor delta gene rearrangement and T early alpha (TEA) expression in immature alpha beta lineage thymocytes: implications for alpha beta/gamma delta lineage commitment.
Périodique
Immunity
Auteur(s)
Wilson A., de Villartay J.P., MacDonald H.R.
ISSN
1074-7613
Statut éditorial
Publié
Date de publication
1996
Peer-reviewed
Oui
Volume
4
Numéro
1
Pages
37-45
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Mature T cells comprise two mutually exclusive lineages expressing heterodimeric alpha beta or gamma delta antigen receptors. During development, beta, gamma, and delta genes rearrange before alpha, and mature gamma delta cells arise in the thymus prior to alpha beta cells. The mechanism underlying commitment of immature T cells to the alpha beta or gamma delta lineage is controversial. Since the delta locus is located within the alpha locus, rearrangement of alpha genes leads to deletion of delta. We have examined the rearrangement status of the delta locus immediately prior to alpha rearrangement. We find that many thymic precursors of alpha beta cells undergo VDJ delta rearrangements. Furthermore, the same cells frequently coexpress sterile T early alpha (TEA) transcripts originating 3' of C delta and 5' of the most upstream J alpha, thus implying that individual alpha beta lineage cells undergo sequential VDJ delta and VJ alpha rearrangements. Finally, VDJ delta rearrangements in immature alpha beta cells appear to be random, supporting models in which alpha beta lineage commitment is determined independently of the rearrangement status at the TCR delta locus.
Mots-clé
Amino Acid Transport Systems, Basic, Animals, Base Sequence, Carrier Proteins/biosynthesis, Carrier Proteins/genetics, Cell Differentiation/genetics, Female, Gene Expression Regulation, Developmental, Gene Rearrangement, delta-Chain T-Cell Antigen Receptor, Membrane Proteins/biosynthesis, Membrane Proteins/genetics, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, alpha-beta/immunology, Receptors, Antigen, T-Cell, gamma-delta/genetics, Receptors, Antigen, T-Cell, gamma-delta/immunology, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/02/2010 11:32
Dernière modification de la notice
20/08/2019 15:55
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