Notch1 is required for neuronal and glial differentiation in the cerebellum.

Details

Serval ID
serval:BIB_D4DF03688781
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Notch1 is required for neuronal and glial differentiation in the cerebellum.
Journal
Development
Author(s)
Lütolf S., Radtke F., Aguet M., Suter U., Taylor V.
ISSN
0950-1991 (Print)
ISSN-L
0950-1991
Publication state
Published
Issued date
2002
Volume
129
Number
2
Pages
373-385
Language
english
Abstract
The mechanisms that guide progenitor cell fate and differentiation in the vertebrate central nervous system (CNS) are poorly understood. Gain-of-function experiments suggest that Notch signaling is involved in the early stages of mammalian neurogenesis. On the basis of the expression of Notch1 by putative progenitor cells of the vertebrate CNS, we have addressed directly the role of Notch1 in the development of the mammalian brain. Using conditional gene ablation, we show that loss of Notch1 results in premature onset of neurogenesis by neuroepithelial cells of the midbrain-hindbrain region of the neural tube. Notch1-deficient cells do not complete differentiation but are eliminated by apoptosis, resulting in a reduced number of neurons in the adult cerebellum. We have also analyzed the effects of Notch1 ablation on gliogenesis in vivo. Our results show that Notch1 is required for both neuron and glia formation and modulates the onset of neurogenesis within the cerebellar neuroepithelium.
Keywords
Animals, Apoptosis, Biological Markers, Cell Differentiation, Cell Survival, Cells, Cultured, Cerebellum/cytology, Cerebellum/embryology, Embryo, Mammalian/anatomy &amp, histology, Embryo, Mammalian/physiology, Gene Expression Regulation, Developmental, Genes, Reporter, Immunohistochemistry, In Situ Hybridization, In Situ Nick-End Labeling, Integrases/genetics, Integrases/metabolism, Intermediate Filament Proteins/metabolism, Membrane Proteins/genetics, Membrane Proteins/metabolism, Mice, Mice, Transgenic, Nerve Tissue Proteins, Neuroglia/cytology, Neuroglia/physiology, Neurons/cytology, Neurons/physiology, Receptor, Notch1, Receptors, Cell Surface, Signal Transduction, Transcription Factors, Viral Proteins/genetics, Viral Proteins/metabolism
Pubmed
Web of science
Create date
28/01/2008 11:39
Last modification date
20/08/2019 15:54
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