Innate immune responses of macrophages and dendritic cells to poxvirus : P199

Details

Serval ID
serval:BIB_D4D3F0146A8C
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Poster: Summary – with images – on one page of the results of a researche project. The summaries of the poster must be entered in "Abstract" and not "Poster".
Collection
Publications
Institution
Title
Innate immune responses of macrophages and dendritic cells to poxvirus : P199
Title of the conference
Annual Joint Meeting of the Swiss Societies for Pneumology, Paediatric Pneumology, Allergology and Immunology, Thoracic Surgery
Author(s)
Delaloye J., Roger T., Esteban M., Steiner-Tardivel Q., Calandra T.
Address
Fribourg, April 17 and 18, 2008
ISBN
1424-7860
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
138
Series
Swiss Medical Weekly
Pages
46S
Language
english
Notes
Background: Attenuated NYVAC and MVA poxvirus strains are currently used for vaccine development against a broad spectrum of diseases. Whilst these vectors have been shown to be immunogenic and safe in humans, the innate immune responses they elicit remain largely unknown.
Aim: To assess the response of human THP-1 cells and mouse dendritic cells to poxviruses infection and to elucidate the role of the Toll-like-receptor (TLR) pathways in that response.
Results: MVA-stimulated THP-1 monocytes secreted large amounts of chemokines (MIP-1, MCP-1, IP-10, RANTES), but low levels of proinflammatory cytokines (TNF, IL-6, IL-1beta). In contrast, NYVAC induced weak production of both cytokines and chemokines in THP-1 cells. Using wild-type and TLR2, TLR4, MyD88 and TRIF deficient mouse dendritic cells, we observed that TLR2-MyD88 was essential for production of chemokines, but dispensable for IFNbeta production, after stimulation with MVA.
Conclusion: MVA triggers innate immune responses of macrophages and dendritic cells via TLR-dependent and TLR-independent pathways. Work is in progress to identify the TLR-independent pathway(s) activated by MVA.
Web of science
Create date
13/10/2009 13:16
Last modification date
20/08/2019 15:54
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