Assessment of retinoblastoma RNA reflux after intravitreal injection of melphalan.

Détails

ID Serval
serval:BIB_D247B1949B80
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Assessment of retinoblastoma RNA reflux after intravitreal injection of melphalan.
Périodique
The British journal of ophthalmology
Auteur(s)
Winter U., Nicolas M., Sgroi M., Sampor C., Torbidoni A., Fandiño A., Chantada G.L., Munier F.L., Schaiquevich P.
ISSN
1468-2079 (Electronic)
ISSN-L
0007-1161
Statut éditorial
Publié
Date de publication
03/2018
Peer-reviewed
Oui
Volume
102
Numéro
3
Pages
415-418
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Intravitreal injection of chemotherapy in retinoblastoma eyes with vitreous seeds may lead to a risk of extraocular tumour dissemination that has not been assessed so far.
To develop a sensitive and clinically feasible technique to assess for potential retinoblastoma cell reflux after intravitreal injection of melphalan.
Filter papers were cut in 6 mm diameter circles and sterilised before use. Eyes with retinoblastoma vitreous seeds (group D, International Classification) received weekly intravitreal melphalan injections (20 µg or 30 µg/dose) followed by cryotherapy as part of local treatment. Immediately after finishing the injection and cryotherapy, filter papers were placed on the injection site and on the cryoprobe tip to assess for the expression of the cone-rod homeobox gene (CRX) by real-time qPCR as a surrogate of retinoblastoma RNA. The assay was developed and validated to determine sensitivity, linearity, recovery, repeatability and reproducibility.
The assay for quantitation of CRX expression was linear in the range of 1 to 1000 cells. The lowest limit of detection was one retinoblastoma cell and allowed to recover 100% of the cell load in external supplementation. A total of 14 eyes received 22 cycles of intravitreal melphalan and were evaluated for potential extraocular tumour cell dissemination using the developed technique. None of the cycles were positive for CRX in samples from the scar or from the cryoprobe tip.
A sensitive and simple method of tumour cell assessment has been developed that can be used in the clinics to assess for potential extraocular dissemination after intravitreal injections to assure its performance.
Mots-clé
Antineoplastic Agents, Alkylating/administration & dosage, Biomarkers, Tumor, Cryotherapy, Homeodomain Proteins/genetics, Humans, Intravitreal Injections, Melphalan/administration & dosage, Neoplasm Seeding, RNA, Neoplasm/analysis, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Retinal Neoplasms/drug therapy, Retinal Neoplasms/genetics, Retinoblastoma/drug therapy, Retinoblastoma/genetics, Retrospective Studies, Trans-Activators/genetics, Tumor Cells, Cultured, experimental laboratory, retina, treatment medical, vitreous
Pubmed
Web of science
Open Access
Oui
Création de la notice
02/11/2017 20:51
Dernière modification de la notice
20/08/2019 15:52
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