Article: article from journal or magazin.
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Recherche de l'amplification du gène HER2: la CISH est-elle une alternative à la technique FISH [HER2 gene amplification assay: is CISH an alternative to FISH?].
Annales De Pathologie
Groupe d'Etude des Facteurs Pronostiques en Immunohistochimie dans le Cancer du Sein
Publication types: Comparative Study ; English Abstract ; Evaluation Studies ; Journal Article ; Review
The HER2 proto-oncogene encodes a transmembrane protein, which is considered to function as a growth factor receptor. Overexpression of this protein found by immunohistochemistry in about 20% of infiltrating breast carcinomas, has a predictive value of response to treatment by trastuzumab, an anti-HER2 humanized monoclonal antibody. Search for HER2 gene amplification is necessary to adapt the immunohistochemical technique quality and also in the cases of delicate analysis or weak overexpression. It is usually carried out by Fluorescence In Situ Hybridization (FISH). A more recent hybridization technique, named CISH because of its chromogenic revelation is an alternative method, which gives highly correlated results with FISH. We present details of this technique, which may be more familiar for the pathologists than FISH, because reading analysis is similar to that of immunohistochemical staining.
Breast Neoplasms/chemistry, Breast Neoplasms/genetics, Carcinoma, Ductal, Breast/chemistry, Carcinoma, Ductal, Breast/genetics, Chromogenic Compounds/analysis, Chromosomes, Human, Pair 17/genetics, DNA Probes, Digoxigenin/analysis, Female, Genes, erbB-2, Humans, In Situ Hybridization/methods, In Situ Hybridization, Fluorescence, Nucleic Acid Amplification Techniques, Specimen Handling
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