Detection of acetylcodeine in urine as an indicator of illicit heroin use: method validation and results of a pilot study.

Détails

ID Serval
serval:BIB_D1A8DBE16DC9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detection of acetylcodeine in urine as an indicator of illicit heroin use: method validation and results of a pilot study.
Périodique
Clinical Chemistry
Auteur(s)
Staub C., Marset M., Mino A., Mangin P.
ISSN
0009-9147
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
47
Numéro
2
Pages
301-307
Langue
anglais
Résumé
BACKGROUND: Acetylcodeine (AC), an impurity of illicit heroin synthesis, has been suggested as an interesting biomarker of illicit heroin use. METHODS: Procedures were developed for quantification of (a) morphine, 6-monoacetylmorphine (6-AM), and codeine in urine and (b) diacetylmorphine and AC in urine. Solid-phase extraction of the analytes was performed, and the extracted analytes were analyzed by selected-ion monitoring with gas chromatography-mass spectrometry. This procedure required prior derivatization with propionic anhydride. RESULTS: Different validation parameters were determined, such as linearity, reproducibility, extraction recoveries, and cutoffs. Seventy-one urine specimens of illicit heroin abusers and 44 urine specimens of subjects in a heroin maintenance program were analyzed. AC was detected in 85.9% of the samples of the first group but not in any of the samples from subjects taking medical heroin. In the two groups, there were 94.4% and 84.1% 6-AM positive urine specimens, respectively. Detection times were determined for AC and codeine by parallel administration of heroin containing various percentages of AC to four voluntary patients in a heroin maintenance program. The measured detection times were 8 and 23 h for AC and codeine, respectively. CONCLUSIONS: These results indicate that, together with detection of 6-AM in urine, AC is a suitable marker of illicit heroin use.
Mots-clé
Biological Markers, Codeine, Gas Chromatography-Mass Spectrometry, Heroin Dependence, Humans, Immunoassay, Reproducibility of Results, Substance Abuse Detection
Pubmed
Web of science
Création de la notice
08/02/2008 18:43
Dernière modification de la notice
03/03/2018 21:38
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