Recent progresses in the phenotypic and functional characterization of human cytolytic T lymphocytes

Details

Serval ID
serval:BIB_CCBA565299D3
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Recent progresses in the phenotypic and functional characterization of human cytolytic T lymphocytes
Journal
Ricerca in Clinica e in Laboratorio
Author(s)
Moretta  A., Pantaleo  G., Mingari  M. C.
ISSN
0390-5748 (Print)
Publication state
Published
Issued date
12/1984
Volume
14
Number
4
Pages
601-12
Notes
Journal Article
Review --- Old month value: Oct-Dec
Abstract
Over the past several years, a number of major advances have been made in our understanding of the phenotypic and functional heterogeneity of human T lymphocytes. Most of these advances have resulted from: 1. the availability of monoclonal antibodies directed to T cell surface antigens; 2. the development of sensitive microassays to measure lymphocyte function in vitro; 3. the refining of T cell cloning technology. The most relevant studies in delineating how human T cell functions have been based on the analysis of T cell clones with specific cytolytic activity. These studies allowed the recent molecular definition of structures serving as specific antigen receptor on T cells as well as the understanding of the role of accessory molecules involved in specific receptor activity such as T3, T8 and T4 antigens. The development of microculture conditions allowing clonal proliferation of all human T lymphocytes, whether resting or activated, has provided a major tool for the precise definition of the frequency and subset distribution of T cells with different functional capabilities. Studies along this line have shown that precursors of cytolytic T lymphocytes (CTL-P) represent about one third of peripheral blood human T lymphocytes; in addition, all T8+ were CTL-P, whereas CTL-P were relatively rare among normal T4+ lymphocytes. As much as 20% cytolytic T lymphocytes were found to be capable to release interleukin-2 and B cell growth factor, thus indicating that cytolytic and helper function may be associated at the single cell level.
Keywords
Antibodies, Monoclonal/immunology Antigens, Surface/*immunology Clone Cells Cytotoxicity, Immunologic Humans Killer Cells, Natural/immunology Lymphocyte Activation Lymphocyte Culture Test, Mixed Lymphocyte Function-Associated Antigen-1 Phenotype Receptors, Antigen, T-Cell/immunology T-Lymphocytes, Cytotoxic/*immunology T-Lymphocytes, Helper-Inducer/immunology
Pubmed
Web of science
Create date
25/01/2008 15:14
Last modification date
20/08/2019 15:47
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